Effects of salt stress on gene expression and activities of POD and PPO in of broad bean (Vicia faba L.)‎ cultivars

Abstract EN

Soil salinity is one of the most significant abiotic stresses for crop plants, including legumes.

The effect of different concentrations of NaCl (0, 25, 50 and 100 mM) on dry weight of shoots and roots, shoot height.

The results indicated that increasing of salt concentration was a significant decrease fresh and dry weight of shoots and roots, The effect of different concentrations of NaCl on peroxidase (POD) and Polyphenol oxidase (PPO) enzymes activities in faba bean (Vicia faba) cultivars were investigated.

POD and PPO activities increased significantly with increasing NaCl concentrations, and the maximal levels increased at 50 mM of NaCl concentration while the activity decreased at high NaCl concentration in all broad bean cultivars except Nubaria1 cultivar.

The optimum NaCl concentration for POD and PPO activities were at 25 mM and 0 mM in all cultivars accept Nubaria1 the high activity was at 50mM throw addition of NaCl concentration in reaction of enzyme.

The level expression of POD gene in lozoda and Nubaria1 were high gene expression at 50 mM, while giza843 and giza3 were high gene expression at 25 mM.

Itay1 was high gene expression at 25 mM.

The level expression of PPO gene in lozoda and giza3 were high gene expression at 25 mM, ,while giza843 and Nubaria1 were high gene expression at 50 mM.

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Itay1 was high gene expression at 100 mM.

lozoda and Itay1 were high gene expression, while giza843 and giza3 were moderately gene expression.

Nobaria1 was low POD gene expression