Induction of salt tolerant mutants of foeniculum vulgare by dimethyl sulphate and their identification using protein pattern and ISSR markers

Abstract EN

This work aimed to study the effect of dimethyl sulphate (DMS) at 0, 1000 and 2000 ppm.

and salt stress at 0, 50 and 100 mM NaCl on the growth, essential oils percentage and composition and antioxidant enzyme of Foeniculum vulgare (fennel).

In addition, to study the ability of DMS in induction salt tolerant mutant and evaluate the genetic variation using protein patterns and six ISSR markers.

Salinity decreased fennel growth and productivity chlorophyll a and b and peroxidase (POD) activity.

It induced a noticeable increase in carotenoids, catalase (CAT) activity, Na+ and lipid peroxidation.

Conversely, DMS alleviated the lethal effect of salt stress on fennel plants especially on the M2 generation.

Two mutants were found to be superior to the control plant grown under salt stress and differed in their essential oil composition.

The dendrogram grouped the two tolerant mutants into one cluster