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Lissencephaly-1 gene is required for establishment of axis formation in drosophila melanogaster
Source
Annals of Agricultural Science, Moshtohor
Issue
Vol. 54, Issue 3 (30 Sep. 2016), pp.621-628, 8 p.
Publisher
Banha University Faculty of Agriculture
Publication Date
2016-09-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Lissencephaly-1gene is known to play many roles during drosophila development, it regulates cyst division, oocyte nucleus migration and control germ stem cell self-renewal hence oocyte formation.
In this study we investigated the differential expression of UAS lis-1 RNAi gene construct under the effect of five GAL4 drivers (Nos GAL4, Nub GAL4, Eve 3+7 GAL4, Kni GAL4, Ftz GAL4)on cuticle development.
Results showed that Nos and Nub GAL4/UAS lis-1 RNAi have strong effect on abdominal segment development.
Meanwhile, Eve 3+7, Kni and Ftz GAL4/UAS lis-1 RNAi showed moderate effect.
Proteomic studies (protein- protein interaction) using bicoid (bcd) and even–skipped antibody were used for determining the effect of Nos GAL4/UAS lis-1 RNAi on the expression of maternal and zygotic genes during developmental process.
Results showed that Nos GAL4/UAS lis-1 RNAi gene affected slightly on the distribution of the bicoid protein.
On the other hand using even-skipped antibody showed alteration in the head length and stripe pattern comparing with control.
The overall data showed that UAS lis-1 RNAi gene play an essential role in drosophila axis formation and can be considered as a zygotic effect.
American Psychological Association (APA)
Muhammad, Shirin A.& al-Shawaf, I. I. S.& Hasan, Abd al-Wahhab& al-Jarhi, Huda A. S.& Fahmi, Kh.. 2016. Lissencephaly-1 gene is required for establishment of axis formation in drosophila melanogaster. Annals of Agricultural Science, Moshtohor،Vol. 54, no. 3, pp.621-628.
https://search.emarefa.net/detail/BIM-820336
Modern Language Association (MLA)
Muhammad, Shirin A.…[et al.]. Lissencephaly-1 gene is required for establishment of axis formation in drosophila melanogaster. Annals of Agricultural Science, Moshtohor Vol. 54, no. 3 (2016), pp.621-628.
https://search.emarefa.net/detail/BIM-820336
American Medical Association (AMA)
Muhammad, Shirin A.& al-Shawaf, I. I. S.& Hasan, Abd al-Wahhab& al-Jarhi, Huda A. S.& Fahmi, Kh.. Lissencephaly-1 gene is required for establishment of axis formation in drosophila melanogaster. Annals of Agricultural Science, Moshtohor. 2016. Vol. 54, no. 3, pp.621-628.
https://search.emarefa.net/detail/BIM-820336
Data Type
Journal Articles
Language
English
Record ID
BIM-820336