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Isolation and identification of rhizobial strains from faba bean nodules
Other Title(s)
عزل و تعريف سلالات الربزوبيا من العقد البكتيرية للفول البلدي
Joint Authors
Abu Talib, Hatim Husayn Yusuf
Abu Ali, Hamid al-Sayyid
Husayn, Muna Husayn Abd al-Fattah
Zaghlul, Rashid Abd al-Fattah
Abd al-Rahman, Hani Muhammad Ahmad
Source
Annals of Agricultural Science, Moshtohor
Issue
Vol. 54, Issue 3 (30 Sep. 2016), pp.591-600, 10 p.
Publisher
Banha University Faculty of Agriculture
Publication Date
2016-09-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
To maximize of faba bean production in the new reclaimed land in Egypt need to isolate and select of effective rhizobial strains.
Thirty rhizobial isolates were collected from the healthy root nodule of faba bean (Vicia faba) grow in different geographic locations and soil properties in Egypt.
Morphological studies, nodulation, salinity tolerance, as well as 16S rDNA partial sequence were used for identification and characterization the obtained rhizobial isolates.
All tested isolates were short rod, Gram negative and most of them were opaque and high viscosity.
According to nodulation, the promising isolates were determined.
The most promising isolates tolerated the high NaCl concentration up to 3.5 and 4%.
Moreover the two isolates are 2 is and Nob3 had a positive growth at 4.5% NaCl concentration (107Rhizobial cell per ml.).
Data showed that both Is 2 and Nob 3 isolates were the best isolates where they gave the highest number of nodules and growth characteristics of the two faba bean varieties.
The 16S rDNA sequencing results revealed that the nearest bacterial species to our isolate M4 was Rhizobium sp.
(KF111868) 94% identity while, M6 isolate was 99% identical to Rhizobium rubi (BBJU0100046).
The sequence was submitted to NCBI website with accession number Gen Bank: KX639721 and KX639722 respectively.
American Psychological Association (APA)
Husayn, Muna Husayn Abd al-Fattah& Zaghlul, Rashid Abd al-Fattah& Abd al-Rahman, Hani Muhammad Ahmad& Abu Talib, Hatim Husayn Yusuf& Abu Ali, Hamid al-Sayyid. 2016. Isolation and identification of rhizobial strains from faba bean nodules. Annals of Agricultural Science, Moshtohor،Vol. 54, no. 3, pp.591-600.
https://search.emarefa.net/detail/BIM-820341
Modern Language Association (MLA)
Husayn, Muna Husayn Abd al-Fattah…[et al.]. Isolation and identification of rhizobial strains from faba bean nodules. Annals of Agricultural Science, Moshtohor Vol. 54, no. 3 (2016), pp.591-600.
https://search.emarefa.net/detail/BIM-820341
American Medical Association (AMA)
Husayn, Muna Husayn Abd al-Fattah& Zaghlul, Rashid Abd al-Fattah& Abd al-Rahman, Hani Muhammad Ahmad& Abu Talib, Hatim Husayn Yusuf& Abu Ali, Hamid al-Sayyid. Isolation and identification of rhizobial strains from faba bean nodules. Annals of Agricultural Science, Moshtohor. 2016. Vol. 54, no. 3, pp.591-600.
https://search.emarefa.net/detail/BIM-820341
Data Type
Journal Articles
Language
English
Record ID
BIM-820341