Development of haploid plants through ovary culture technique in wheat (Triticum aestivum L.)‎

Abstract EN

This research was done at department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute( GEBRI ), Sadat City university, Menufyia, Egypt from ٢٠٠٩ to ٢٠١٤ years to study the effect of four factors affected callus induction of ovary cultur i.e.(cold pretreatment, chemical pretreatments, growth regulators and spikes sources.

and establishment a technique to produce haploid plants.

Three wheat varieties were used, Sids ١, Gemmeiza ٩ and Giza ١٦٨.

The results indicated that, chemical pretreatments by sterilization substances (Ethanol alcohol ٧٠% or Mercurec chloride ١g/L or Thiourea ٢g/L) were very important and essential to induce calli from ovaries.

The combination between (١ and ٢ mg /L ٢,٤-D ) and (٠ , ٠.١ and ٠.٢ mg/L kinetien) gave the highest response of callus induction and plant regeneration percentages.

The estimates were increased and developed with increasing the cultured area which the spiks were collected from it.

The estimates were varied with varieties.