Isolation and expression of dehydrin and dreb2 transcription factor genes in some wheat (Triticum asetivum L.)‎ cultivars .

Abstract EN

Anther culture was utilized to develop haploid plants in wheat .This study was carried out at Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute ( G E B R I ) from ٢٠٠٩ to ٢٠١٤ years .

Three varieties of wheat were used , Sids١, Gemmeiza ٩ and Giza ١٦٨.

The studied factors were, cold and chemical pretreatments, growth regulators concentrations of ٢,٤-D and kinetin and effect of source of spikes.

The results indicated that the cold pretreatment had no effect on anther response of three wheat varieties, the chemical pretreatments by sterilization substances (ethanol alcohol ٧٠% or mercurec chloride ١g /L or thiourea ٢ g /L) were very important and essential to induce callus from anther.

The three treatments of (١mg /L ٢,٤-D ), ( ٢ mg /L ٢,٤-D) and (٢ mg /L ٢,٤-D + ٠.١ mg /L kinetin ) gave the highest response of callus induction and plant regeneration.

Androgenesis improved with increasing the cultured area which the spikes were collected from it.