Thin layer chromatographic investigation of some veterinary drugs

Other Title(s)

التحري عن بعض أدوية البيطرية بواسطة استشراب الطبقة الرقيقة

Joint Authors

Yahya, Basil Muhammad
Hamad, Zaynah Sattam

Source

المجلة العراقية للعلوم البيطرية

Publisher

University of Mosul College of Veterinary Medicine

Publication Date

2012-12-31

Country of Publication

Iraq

No. of Pages

5

Main Subjects

Zoology
Veterinary Medicine

Topics

• Drugs
• Thin layer chromatography

English Abstract

simple technique, using solvent extraction and thin layer chromatography for the detection and separation of a number of commonly prescribed veterinary drugs was studied.

Blood and urine have been considered as possible biological samples taken from the animals.

The extraction of each sample was done according to the type of the drug as it is acidic or basic, then the extract was spotted on TLC plate by capillary tube after lining the starting line first.

Separations of the components of the samples were developed after running the plate in a tight lid tank containing solvent (mobile phase).

The identification of separated spots were carried out after lining the end line for the solvent front then by spraying the plate with mercurous nitrate reagent for acidic drugs and acidic iodoplatinate or potassium permanganate reagent for basic drugs.

The rate of flow value (Rf) for each compound was measured and the color of spots were determined.

This work provides a simple and rapid qualitative technique for the detection of veterinary drugs used at therapeutic doses and in overdosage.

Data Type

Conference Papers

Record ID

BIM-830834

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