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Prevalence and molecular analysis of occult hepatitis B virus infection isolated in a sample of cryptogenic cirrhosis patients in Iran
Joint Authors
Anvari, Fatimah Akhavan
Norouzi, Mahdi
Mahabadi, Mustafa
Jazayeri, Sayyid Muhammad
Alavian, Sayyid Muayyad
Source
Issue
Vol. 29, Issue 2 (30 Apr. 2014), pp.92-96, 5 p.
Publisher
Publication Date
2014-04-30
Country of Publication
Oman
No. of Pages
5
Main Subjects
Abstract EN
The aims of this study are to investigate the prevalence of occult hepatitis B virus infection among patients with cryptogenic cirrhosis and to analyze the relationship between surface protein variability and occult hepatitis B virus infection, which may be related to the pathogenesis of occult hepatitis B virus infection in cryptogenic cirrhosis.
Occult hepatitis B virus infection is a wellrecognized clinical entity characterized by the detection of hepatitis B virus DNA in serum and/or liver in the absence of detectable hepatitis B virus surface antigen, with or without any serological markers of a past infection.
Methods: Sera from patients with cryptogenic chronic liver disease were tested for hepatitis B virus DNA using both real-time and nested PCR.
In the detected hepatitis B virus DNA samples, the surface gene was analyzed for mutations.
Results: Hepatitis B virus DNA was detected in 38% of patients, all of whom had a viral load below 10,000 copies/mL.
All hepatitis B virus belonged to genotype D.
There were no significant associations between occult hepatitis B virus infection status and age, gender, ALT/AST levels, viral load or serologic markers of previous hepatitis B virus infection.
There were 14 mutations found in 5 patients; 6 were in the major hydrophilic region, of which 4 were Y134F assigning for the "a" determinant region.
All patients who acquired Y134F contained S207R (within HLA-A2-restricted CTL epitope) as a combination.
Conclusion: Hepatitis B virus surface antigen variants may arise as a result of natural selection to evade the immune surveillance of the infected host, and subsequently may go undetected by conventional hepatitis B virus surface antigen screening tests.
Etiological diagnosis of cryptogenic cirrhosis is significantly underestimated with current serology testing methods alone.
American Psychological Association (APA)
Anvari, Fatimah Akhavan& Alavian, Sayyid Muayyad& Norouzi, Mahdi& Mahabadi, Mustafa& Jazayeri, Sayyid Muhammad. 2014. Prevalence and molecular analysis of occult hepatitis B virus infection isolated in a sample of cryptogenic cirrhosis patients in Iran. Oman Medical Journal،Vol. 29, no. 2, pp.92-96.
https://search.emarefa.net/detail/BIM-831728
Modern Language Association (MLA)
Anvari, Fatimah Akhavan…[et al.]. Prevalence and molecular analysis of occult hepatitis B virus infection isolated in a sample of cryptogenic cirrhosis patients in Iran. Oman Medical Journal Vol. 29, no. 2 (2014), pp.92-96.
https://search.emarefa.net/detail/BIM-831728
American Medical Association (AMA)
Anvari, Fatimah Akhavan& Alavian, Sayyid Muayyad& Norouzi, Mahdi& Mahabadi, Mustafa& Jazayeri, Sayyid Muhammad. Prevalence and molecular analysis of occult hepatitis B virus infection isolated in a sample of cryptogenic cirrhosis patients in Iran. Oman Medical Journal. 2014. Vol. 29, no. 2, pp.92-96.
https://search.emarefa.net/detail/BIM-831728
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 95-96
Record ID
BIM-831728