Assessment of ISNAG fluorimeter (total fluorescence measurements at+90° &-90° using four solar cell on each side for 100mm distance at 2mm path length)‎ with well-known fluorescent molecules via CFIA

Abstract EN

Two well-known fluorescent molecules: fluorescein sodium salt (FSS) and 2,7- dichloro fluorescein (DCF) were tried to prove the efficiency, trustability and repeatability of ISNAG fluorimeter by using discrete and continuous flow injection analysis modes.A linear range of 0.002-1 mmol/L for FSS and 0.003-0.7 mmol/L was for DCF, with LOD 0.0018 mmol/L and 0.002 mmol/L for FSS and DCF respectively, were obtained for discrete mode of analysis.

While the continuous mode gave a linear range of 0.002-0.7 mmol/L and 0.003-0.5 mmol/L for FSS and DCF respectively, the LOD were 0.0016mmol/L and 0.0018 mmol/L for FSS and DCF respectively.

The results were compared with classical method at variable λex for both fluorescent molecules at 95% confidence level.

The comparison data shows that ISNAG fluorimeter is the choice with excellent extended detection and a wider applicability.