Increased transforming growth factor-ß and interleukin-17 transcripts in peripheral blood of breast cancer patients with different clinical stages

Abstract EN

Background: Currently, cancer as major problem around the world threatens human health and has a high incidence in developing countries.

Many reports have showed that there is a dysfunction in the immune system of cancer patients.

Interleukin-17 (IL-17) and transforming growth factor (TGF--) are increased in the generated through cancers, and IL-17 is considered as a pro-inflammatory cytokine; IL-17 is an angiogenic factor and promotes tumor growth.

Materials and Methods: In this study TGF and IL-17, mRNA gene expression has been measured in peripheral blood of 35 breast cancer patients and 35 normal age-matched women using quantitative real-time polymerase chain reaction method with master mix reaction containing SYBER Green.

actin gene was used as housekeeping gene.

Results: Our data demonstrated a significant upregulation of TGFand IL-17 gene expression (P-0.05) in patient’s peripheral blood compared to normal healthy control.

Levels of genes expression were higher in the breast cancer group compared to the control group.

Conclusion: TGFand IL-17 expression could be used as a diagnostic marker for detecting breast cancer.