Molecular analysis of klebsiella pneumoniae isolated from UTI patients in Erbil City
Joint Authors
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 30, Issue 3 (30 Jun. 2018), pp.1-9, 9 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
Fifty isolates of Klebsiella pneumoniae were identified using cultural, morphological and biochemical characteristics.
Three-hundred and fifty urine samples were collected from patients with urinary tract infection admitted to Hawler Teaching, Rizgary, Raparin and West Emergency hospitals in Erbil city during the period of 2nd of June to 5th of October 2012.
Antibiotic sensitivity testing performed for all isolates using amikacin (AK30), ampicillin (AMP10), aztreonam (ATM30), Cefixime (CFM5), Ceftriaxone (CI30), Ciprofloxacin (CIP30), Cefoxitin (CN30), Trimethoprim/sulfamethoxazole (COT25), Cefotaxime (CTX5), Gentamicin (GEN10), Imipenem (IPM10), Nalidixic acid (NA30), Nitrofurantoin (NIT300), Norfloxacin (NOR10) and Trimethoprim (TMP5).
The resistance percentage of the isolates were (8, 100, 64, 52, 60, 20, 86, 50, 60, 34, 0, 24, 58, 24 and 60) %, respectively.
Plasmid profiling was done for all isolates and it was revealed that 48% contained one plasmid, 44% possessed two plasmids, 6% had three plasmids and 2% of the isolates contained four plasmids.
The process of transformation was done for the laboratory strain Escherichia coli DH5α by using purified plasmid of the most resistant isolate (K50) to determine the location of the genes which are responsible for resistance to antibiotics in that isolate.
The transformed colonies appear to be resistant to the following antibiotics (AMP10, CFM5 and CTX5) which indicated that the genes encoding resistance to these antibiotics were located on transferrable plasmid.
American Psychological Association (APA)
Tawgozi, Fayruz H. Abd Allah& Amin, Bushra K.. 2018. Molecular analysis of klebsiella pneumoniae isolated from UTI patients in Erbil City. ZANCO Journal of Pure and Applied Sciences،Vol. 30, no. 3, pp.1-9.
https://search.emarefa.net/detail/BIM-832797
Modern Language Association (MLA)
Tawgozi, Fayruz H. Abd Allah& Amin, Bushra K.. Molecular analysis of klebsiella pneumoniae isolated from UTI patients in Erbil City. ZANCO Journal of Pure and Applied Sciences Vol. 30, no. 3 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-832797
American Medical Association (AMA)
Tawgozi, Fayruz H. Abd Allah& Amin, Bushra K.. Molecular analysis of klebsiella pneumoniae isolated from UTI patients in Erbil City. ZANCO Journal of Pure and Applied Sciences. 2018. Vol. 30, no. 3, pp.1-9.
https://search.emarefa.net/detail/BIM-832797
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 7-9
Record ID
BIM-832797