Immunophenotypic analysis of bone marrow derived mesenchymal stem cells in albino rats

Abstract EN

Background and Objectives Mesenchymal stem cells (MSCs) are pluripotent adult stem cells residing within the bone marrow (BM) capable of self renewal, production of large number of differentiated progeny and regeneration of tissue.

This study aimed to isolating and culturing of the BM-MSCs from albino rats and detecting of the phenotypic nature of rat MSCs.

Methods The BM was collected from young male white rats and separated by gradient centrifugation, then the mononuclear cells (MNCs) were retrieved from the buffy layer and cultured in Modified Eagle,s Medium (MEM) supplemented with 10% Fetal calf serum (FCS) and incubated at 37Cº and 5% CO2.

Then immunophenotypic analysis of MSCs were detected by using CD markers (CD 11b, CD34, CD71 and CD90).

Results After 10-12 days of primary culture, the MNCs derived MSCs was duplicating rapidly and showed fibroblast like morphology appearance.

By the end of the second week the adherent cells formed monolayer which expanded by two passages.

The results of immunophenotypic analysis of MSCs indicated that most of them express positive response for CD71, CD90 and negative response for CD11b, CD34.

Conclusion: The results of the present study indicate the major population of adherent cells are MSCs and these cells are positive for CD71, CD90 the MSCs specific markers, and negative for heamtopoeitic specific markers (CD11b, CD34) .