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Derivative spectrophotometric determination of florfenicol in chicken samples
Joint Authors
Rashid, Shannar M.
Fakhri, Nabil A.
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 29, Issue 6 (31 Dec. 2017), pp.49-58, 10 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract EN
A simple, rapid, and reliable derivative spectrophotometric method for determination of florfenicol was developed.
Florfenicol belongs to amphenicol group antibiotics, the most widespread antibiotics used in poultry.
Antibiotic residues in edible tissues of food-producing animals contribute to several reasons, such as a withdrawal period, overdose and long acting drugs.
The analysis of the derivative spectra was done through the determination of the derivative values, using one of the four graphical methods, which included peak–to-baseline (peak height), peak-to- peak, and area under peak.
Under the optimum experimental conditions for the first derivative spectrophotometric method, a good proportionality was found between florfenicol concentration and peak amplitude at 218.6 and 233.8 nm, over the concentration range of 1.0-53 μg/ml, 0.5-53 μg/ml, intervals of 211.2-224 nm and 224-249.2 nm, over the concentration range 0.7-53 μg/ml and 0.3-53 μg/ml, with detection limit of 0.7, 0.3, 0.1 μg/ml.
The proposed method was applied for the determination of florfenicol in chicken tissues, thigh and arms, liver and kidney with recovery percentage of 95.81-114%.
American Psychological Association (APA)
Rashid, Shannar M.& Fakhri, Nabil A.. 2017. Derivative spectrophotometric determination of florfenicol in chicken samples. ZANCO Journal of Pure and Applied Sciences،Vol. 29, no. 6, pp.49-58.
https://search.emarefa.net/detail/BIM-833907
Modern Language Association (MLA)
Rashid, Shannar M.& Fakhri, Nabil A.. Derivative spectrophotometric determination of florfenicol in chicken samples. ZANCO Journal of Pure and Applied Sciences Vol. 29, no. 6 (2017), pp.49-58.
https://search.emarefa.net/detail/BIM-833907
American Medical Association (AMA)
Rashid, Shannar M.& Fakhri, Nabil A.. Derivative spectrophotometric determination of florfenicol in chicken samples. ZANCO Journal of Pure and Applied Sciences. 2017. Vol. 29, no. 6, pp.49-58.
https://search.emarefa.net/detail/BIM-833907
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 57-58
Record ID
BIM-833907