Curing of some antibiotic resistance by the action of sodium dodecyl sulphate and ethidium bromide in Pseudomonas aeruginosa Isolated from urine of human, cow meat and horses wound
Other Title(s)
تحييد بعض المورثات المقاومة للمضادات الحيوية بواسطة كبريتات دوديسيل الصوديوم و الاثيديوم برومايد في جرثومة الزوائف الزنجارية المعزولة من إدرار الإنسان، لحوم الأبقار و جروح الخيول
Author
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 8, Issue 2 (31 Dec. 2017), pp.115-123, 9 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
Thirty seven isolates of Pseudomonas aeruginosa Twenty-two isolates were collected from urine in Al-Diywania teaching hospital, eleven isolates from cow meat and four isolates from wound horses.
They were identified using the Vitek 2, for detecting characteristics of the biochemical tests and morphological appearance.
All isolates resisted to Nalidixic acid, Oxacillin ,Vancomycin, Ampicillin, and Ciprofloxacin , while show sensitive to Kanamycin and Tetracycline.
The minimum inhibitory concentrations (MICs) of two curing agents, sodium dodecyl sulphate (SDS) and ethidium bromide, used in this study were determined and the results indicated that the curing percentage and efficiency of each curing agent was determined.
From treatment with 700 μg/ml ethidium bromide, it was observed that no cured cells were obtained for all antibiotics used and obtained for all antibiotics used by SDS at a concentration of (1, 0.9, 0.8)% (W/V) where had no effect on plasmid curing and bacterial cells complete lysis, the isolates appeared sensitive to (0.5,0.6 and 0.7)% concentration of SDS were Nalidixic acid 10mg was (35.1, 94.5 and 100) %, Vancomycin 30mg ( 59.4, 86.4 and 100) %, Kanamycin 30mg was (97.2, 100,100)% and Tetracycline 30mg was (75.6, 100 and 100)% respectively , no there sensitivity for each Oxacillin 30mg , Ciprofloxacin 5mg and Ampicillin 10mg.
American Psychological Association (APA)
al-Hasnawi, Zaynah Fuad Salih. 2017. Curing of some antibiotic resistance by the action of sodium dodecyl sulphate and ethidium bromide in Pseudomonas aeruginosa Isolated from urine of human, cow meat and horses wound. Kufa Journal for Veterinary Medical Sciences،Vol. 8, no. 2, pp.115-123.
https://search.emarefa.net/detail/BIM-834011
Modern Language Association (MLA)
al-Hasnawi, Zaynah Fuad Salih. Curing of some antibiotic resistance by the action of sodium dodecyl sulphate and ethidium bromide in Pseudomonas aeruginosa Isolated from urine of human, cow meat and horses wound. Kufa Journal for Veterinary Medical Sciences Vol. 8, no. 2 (Dec. 2017), pp.115-123.
https://search.emarefa.net/detail/BIM-834011
American Medical Association (AMA)
al-Hasnawi, Zaynah Fuad Salih. Curing of some antibiotic resistance by the action of sodium dodecyl sulphate and ethidium bromide in Pseudomonas aeruginosa Isolated from urine of human, cow meat and horses wound. Kufa Journal for Veterinary Medical Sciences. 2017. Vol. 8, no. 2, pp.115-123.
https://search.emarefa.net/detail/BIM-834011
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 122-123
Record ID
BIM-834011