Detection the genetic relation between several fungal isolates by using a universal ITS primers
Other Title(s)
دراسة العلاقة الوراثية بين عدد من العزلات الفطرية باستخدام بادئات لمنطقة المستنسخات الداخلية
Joint Authors
Habib, Nihad
al-Rifiai, Muhammad Muhammad
Samakah, Haydar Mahmud Ali
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 8, Issue 2 (31 Dec. 2017), pp.170-178, 9 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
mary The goal of this study is to evaluate the internal transcribed spacer regions (ITS) of the rDNA to detect a phylogenetic relationshep based on PCR technique between four isolates of Trichoderma viride, Chaetumium elatum and tow isolates (pathogen and non-pathogen strains) of Rhizoctonia solani obtained from tomato plant rhizosphere, rice plant rhizosphere and root rotted tomato plants fields of Al-Najaf province, Iraq.
Internal transcribed spacer regions (ITS) of the ribosomal DNA was amplified by polymerase chain reaction (PCR) technique using a set of common universal primers for ITS1, ITS3 and ITS4 regions.
Two of three different random primers examined (primers 3 and 4) reflected a specific amplicons within Rhizoctonia solani(1), Chaetumium elatum , and Trichoderma viride, while the primer 1 did not give any PCR end product with all of tested isolates, while Rhizoctonia solani(2) isolates did not revealed any band detected in PCR technique with all primers, also data showing a diferent degree of genetic similarity depanding on PCR data analysis using UVIBand softwear.
Results of this study indicate that ITS set of primers can used to find a genetic relation between fungi.
American Psychological Association (APA)
Habib, Nihad& Samakah, Haydar Mahmud Ali& al-Rifiai, Muhammad Muhammad. 2017. Detection the genetic relation between several fungal isolates by using a universal ITS primers. Kufa Journal for Veterinary Medical Sciences،Vol. 8, no. 2, pp.170-178.
https://search.emarefa.net/detail/BIM-834018
Modern Language Association (MLA)
Samakah, Haydar Mahmud Ali…[et al.]. Detection the genetic relation between several fungal isolates by using a universal ITS primers. Kufa Journal for Veterinary Medical Sciences Vol. 8, no. 2 (Dec. 2017), pp.170-178.
https://search.emarefa.net/detail/BIM-834018
American Medical Association (AMA)
Habib, Nihad& Samakah, Haydar Mahmud Ali& al-Rifiai, Muhammad Muhammad. Detection the genetic relation between several fungal isolates by using a universal ITS primers. Kufa Journal for Veterinary Medical Sciences. 2017. Vol. 8, no. 2, pp.170-178.
https://search.emarefa.net/detail/BIM-834018
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 176-178
Record ID
BIM-834018