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Molecular characterization of cryptosporidium spp. in sheep and goat in Al-Qadisiyah Province Iraq
Other Title(s)
التشخيص الجزيئي للأبواغ الخبيئة في الأغنام و الماعز في محافظة القادسية العراق
Joint Authors
al-Khalidi, Mansur Jadan Ali
Hamad, Wiam Abbas
Source
Iraqi Journal of Veterinary Medicine
Issue
Vol. 41, Issue 2 (31 Dec. 2017), pp.31-37, 7 p.
Publisher
University of Baghdad College of Veterinary Medicine
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
The present study was conducted during the period from September 2015 until February 2016.
100 fecal samples were collected from 60 sheep and 40 goats for diagnosis of Cryptosporidium parasite from diverse areas in al-Qadisiyah province.
The study amid to know the genetic characters of Cryptosporidium spp.
parasite by using a molecular technique such as the nested polymerase chain reaction and DNA sequencing analyzed by phylogenetic tree to identify the parasite species.
This study was done on the sheep and goat at first time in the middle region of Iraq and the identified species were recorded in NCBI-Genbank database.
In sheep, the results of positive infected samples was (40 %) while, in the goats were (32.5 %), the DNA sequencing and phylogenetic analysis method based on small ribosomal RNA gene (18s rRNA) for Cryptosporidium species typing.
The results were conducted by Neighbor-Joining phylogenetic tree analysis method and the 18s rRNA gene sequences were confirmed by using NCBI-BLAST data analysis in order to compare with NCBI submitted selected references isolates of (18s ribosomal RNA) gene in Cryptosporidium spp.
parasites.
Our finding in present study appeared to follow spp.
(C.
parvum, C.
hominis, C.
andersoni, C.
ubiquitum, C.
xiaio and C.
suis).
These identified species which primary affected sheep and goats as mentioned in previous studies when compared with newly Iraq isolates strains.
American Psychological Association (APA)
al-Khalidi, Mansur Jadan Ali& Hamad, Wiam Abbas. 2017. Molecular characterization of cryptosporidium spp. in sheep and goat in Al-Qadisiyah Province Iraq. Iraqi Journal of Veterinary Medicine،Vol. 41, no. 2, pp.31-37.
https://search.emarefa.net/detail/BIM-834090
Modern Language Association (MLA)
al-Khalidi, Mansur Jadan Ali& Hamad, Wiam Abbas. Molecular characterization of cryptosporidium spp. in sheep and goat in Al-Qadisiyah Province Iraq. Iraqi Journal of Veterinary Medicine Vol. 41, no. 2 (2017), pp.31-37.
https://search.emarefa.net/detail/BIM-834090
American Medical Association (AMA)
al-Khalidi, Mansur Jadan Ali& Hamad, Wiam Abbas. Molecular characterization of cryptosporidium spp. in sheep and goat in Al-Qadisiyah Province Iraq. Iraqi Journal of Veterinary Medicine. 2017. Vol. 41, no. 2, pp.31-37.
https://search.emarefa.net/detail/BIM-834090
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 36-37
Record ID
BIM-834090