Genotyping of fusa gene from clinical isolates acinetobacter baumannii in Baghdad
Other Title(s)
النمط الوراثي لجين fusA من عزلات سريرية لبكتريا Acinetobacter baumannii في بغداد
Author
Source
Issue
Vol. 15, Issue 1 (31 Mar. 2018), pp.37-42, 6 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2018-03-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
This study aims at detecting the differences in genotyping of coding region fusA gene in clinical isolates of Acinetobacter baumannii from Baghdad, Iraq.
Collected two hundred clinical samples (50 samples from urine, 50 samples from wound, 50 samples from sputum and 50 samples from otitis infections).
Laboratory diagnosis for bacterial isolates carried out by some biochemical tests and confirmed by using VITEK- 2 compact system.
The results appeared that twenty isolates of Acinetobacter baumannii in all these samples.
Genotyping study was performed of coding region fusA gene of the extracted genome of all bacterial isolates and used specific primers in achieved amplification process of this target gene.
DNA sequencing of this gene and alignment of sequencing in NCBI was achieved and drew phylogenetic tree by using Geneious 9 software among locally isolates alone and then among locally isolates and high identity global isolates in GenBank.
The results in phylogenetic tree of fusA gene in locally isolates showed 4 groups of isolates included more than one source of isolation.
The results in phylogenetic tree of the locally and global isolates showed that are four different groups and each group included some locally isolates and global isolates except group A (AE_22, AE_26) and group E (AE_35, AE_32, AE_33) that not identity with global isolates.
The nucleotides sequence of fusA gene from localized isolate (AE_35) was registered in national GenBank under accession number (LOCUS KY818057) and protein ID "ARV90995.1.
American Psychological Association (APA)
Hamzah, Ala Salim. 2018. Genotyping of fusa gene from clinical isolates acinetobacter baumannii in Baghdad. Baghdad Science Journal،Vol. 15, no. 1, pp.37-42.
https://search.emarefa.net/detail/BIM-834151
Modern Language Association (MLA)
Hamzah, Ala Salim. Genotyping of fusa gene from clinical isolates acinetobacter baumannii in Baghdad. Baghdad Science Journal Vol. 15, no. 1 (2018), pp.37-42.
https://search.emarefa.net/detail/BIM-834151
American Medical Association (AMA)
Hamzah, Ala Salim. Genotyping of fusa gene from clinical isolates acinetobacter baumannii in Baghdad. Baghdad Science Journal. 2018. Vol. 15, no. 1, pp.37-42.
https://search.emarefa.net/detail/BIM-834151
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 41-42
Record ID
BIM-834151