Assessment of the antibacterial activity of macroalgae Cladophora crispata extract against extended spectrum beta-lactamase producing escherichia coli isolated from diarrheic children

Abstract EN

Background: Diarrhea is a major cause of morbidity and mortality in children under 5 years old in developing countries.

Objective: This study designed to investigate the occurrence of extended spectrum beta-lactamase producing Escherichia coli (E.

coli ) as etiological infectious agents of diarrhea in children, assessment of their susceptibility/resistance to antibiotics, and to evaluate the antibacterial activity of the macroalgae Cladophora crispata extract towards these bacteria .

Materials and Methods: 150 diarrheic stool samples were collected from children less than 5 years and cultured.

The isolated bacteria were subjected to various identification and biochemical tests.

Then all the isolated bacteria (E.

coli ) were subjected to ESBL-producing screening by double-disc synergy test, the antibacterial activity of algal extract and antibiotic susceptibility test against tested bacteria were determined by using agar well diffusion and disc diffusion methods respectively .

Results: Out of total 150 samples, only 18 isolates revealed the presence of extended spectrum β-lactamase producing E.

coli, which showed highly susceptibility to gentamicin and amikacin, Cladophora crispata extract revealed a strong antibacterial activity against ESBL-producing E.

coli.

Conclusion: Cladophora crispata extract showed a strong antibacterial activity against ESBL-producing E.

coli which can be exploited as source of antibacterial drugs to control and treatment diarrheal infection caused by these bacteria.