Molecular diagnosis of Shigella, salmonella and campylobacter by multiplex real-time PCR in stool culture samples in Ouagadougou (Burkina Faso)
Joint Authors
Bisseye, Cyrille
Sawadogo, Salfo
Compaore, Tegwinde Rebeca
Ouattara, Abu Bakr S.
Ouermi, Djeneba
Djigma, Florencia W.
Simpore, Jacques
Diarra, Birama
Source
Sudan Journal of Medical Sciences
Issue
Vol. 12, Issue 3 (30 Sep. 2017), pp.163-173, 11 p.
Publisher
Omdurman Islamic University Faculty of Medicine
Publication Date
2017-09-30
Country of Publication
Sudan
No. of Pages
11
Main Subjects
Abstract EN
Background : Bacteriological diagnosis of Campylobacter spp, Salmonella spp and Shigella spp could be necessary in case of infectious gastroenteritis syndrome.
The objective of this study was to diagnose concomitantly the three enteropathogenic bacteria by multiplex Real-Time PCR in stool culture samples in Ouagadougou (Burkina Faso).
Materials and Methods : The study was conducted from February 5th to March 9th, 2013.
Two hundred stool samples were received during the study period.
The bacteria were identified by bacterial culture following by multiplex Real-Time PCR.
Results : Shigella spp and Campylobacter spp were sought by culture in all 200 samples.
Enteropathogenic E.
coli was sought only in 37 samples from all children under 2 years old.
The bacterial culture was positive in 12 stool samples.
Shigella spp and Salmonella spp.
were isolated respectively in 5 (2.5%) and 3 samples (1.5% ).
Enteropathogenic E.
coli was isolated in 10.8 % (4/37) of the samples tested.
The multiplex real-time PCR identified bacteria in 20 patients, including 17 cases of Shigella spp., 1 case of Salmonella spp.
and 2 cases of Campylobacter spp.
Conclusions : This study has highlighted the low frequency of 3 sought bacterial genera in stool samples.
It has also demonstrated a significant difference between the culture and the multiplex Real-Time PCR method in the diagnosis of Shigella.
American Psychological Association (APA)
Sawadogo, Salfo& Diarra, Birama& Bisseye, Cyrille& Djigma, Florencia W.& Ouermi, Djeneba& Simpore, Jacques…[et al.]. 2017. Molecular diagnosis of Shigella, salmonella and campylobacter by multiplex real-time PCR in stool culture samples in Ouagadougou (Burkina Faso). Sudan Journal of Medical Sciences،Vol. 12, no. 3, pp.163-173.
https://search.emarefa.net/detail/BIM-834476
Modern Language Association (MLA)
Ouermi, Djeneba…[et al.]. Molecular diagnosis of Shigella, salmonella and campylobacter by multiplex real-time PCR in stool culture samples in Ouagadougou (Burkina Faso). Sudan Journal of Medical Sciences Vol. 12, no. 3 (2017), pp.163-173.
https://search.emarefa.net/detail/BIM-834476
American Medical Association (AMA)
Sawadogo, Salfo& Diarra, Birama& Bisseye, Cyrille& Djigma, Florencia W.& Ouermi, Djeneba& Simpore, Jacques…[et al.]. Molecular diagnosis of Shigella, salmonella and campylobacter by multiplex real-time PCR in stool culture samples in Ouagadougou (Burkina Faso). Sudan Journal of Medical Sciences. 2017. Vol. 12, no. 3, pp.163-173.
https://search.emarefa.net/detail/BIM-834476
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 170-173
Record ID
BIM-834476