Obtaining heterokaryons following electrical fusion between mesophyll and transformed hairy roots protoplasts of sugarbeet

Joint Authors

al-Nimah, Q. S.
al-Mallah, M. K.

Source

Mesopotamia Environment Journal

Publisher

University of Babylon Environmental Research and Studies Center

Publication Date

2018-12-31

Country of Publication

Iraq

No. of Pages

8

Main Subjects

Botany

English Abstract

Eleven enzyme mixtures consisted from various cell wall degrading enzymes were succeed in isolation of protoplasts from leaf mesophyll of axenic seedlings of sugarbeet, Beta vulgaris L..

Also, some of these enzymatic mixture support the isolation of protoplasts from the adventitious hairy roots produced as a result of sugarbeet explants inoculation with Agrobacterium rhizogenes R1601.

Somatic hybridization between the two types of protoplasts took place through electrofusion and approach 39% under conditions of 1MHz, 1000 Vcm-1, 2 pulses, 1.5 msec./pulse

Data Type

Conference Papers

Record ID

BIM-835431

American Psychological Association (APA)

al-Nimah, Q. S.& al-Mallah, M. K.. 2018-12-31. Obtaining heterokaryons following electrical fusion between mesophyll and transformed hairy roots protoplasts of sugarbeet. International Conference of Science and Art (2 : 2018 : Babylon, Iraq). . Vol. 5, Special issue E (2018), pp.107-114.Babylon Iraq : University of Babylon Environmental Research and Studies Center.
https://search.emarefa.net/detail/BIM-835431

Modern Language Association (MLA)

al-Nimah, Q. S.& al-Mallah, M. K.. Obtaining heterokaryons following electrical fusion between mesophyll and transformed hairy roots protoplasts of sugarbeet. . Babylon Iraq : University of Babylon Environmental Research and Studies Center. 2018-12-31.
https://search.emarefa.net/detail/BIM-835431

American Medical Association (AMA)

al-Nimah, Q. S.& al-Mallah, M. K.. Obtaining heterokaryons following electrical fusion between mesophyll and transformed hairy roots protoplasts of sugarbeet. . International Conference of Science and Art (2 : 2018 : Babylon, Iraq).
https://search.emarefa.net/detail/BIM-835431