تقصي جين V-ATPase subunit C لدى سلالة سورية لليشمانية المدارية
Other Title(s)
Investigation of V-ATPase subunit C gene in Syrian strain of Leishmania Tropica
Joint Authors
العموري، مصطفى
محمد معروف
عرابي، أميرة
Source
Issue
Vol. 8, Issue 5 (31 Jan. 2018)6 p.
Publisher
Syrian Clinical Laboratory Association
Publication Date
2018-01-31
Country of Publication
Syria
No. of Pages
6
Main Subjects
Abstract EN
Cutaneous Leishmaniasis is considered endemic disease in Syria.
The spread of Leishmaniasis has significantly increased since 2011 in Syria, and most infections are caused by Leishmania tropica.
Current studies focus on finding out efficient vaccine that prevents the infection with leishmania.
DNA vaccines are considered a promising approach to protect from this infection.
V-ATPase enzyme has essential role in the life cycle of parasite, where its function to acidify intracellular compartments in eukaryotic cells.
In this study we have chosen V-ATPase subunit C, which is a part of this enzyme, to design DNA vaccine against leishmania tropica infections.
Our study aimed to investigate ATPase subunit C gene in Syrian strain of L.tropica (LCED syrian 01) promastigotes and their expression.
DNA and RNA were isolated from these parasites, synthesized cDNA, designed the necessary primers for gene amplification and optimization of PCR conditions.
The existence of this gene in L.tropica promastigotes has been proved and its size was approximately 675 bp.
Expression of this gene was also proved in these parasites, through proving its existence in extracted RNA of Leishmania tropica promastigotes and amplfication the complementary DNA of this gene.
American Psychological Association (APA)
عرابي، أميرة ومحمد معروف والعموري، مصطفى. 2018. تقصي جين V-ATPase subunit C لدى سلالة سورية لليشمانية المدارية. مجلة التشخيص المخبري،مج. 8، ع. 5.
https://search.emarefa.net/detail/BIM-835812
Modern Language Association (MLA)
عرابي، أميرة....[و آخرون]. تقصي جين V-ATPase subunit C لدى سلالة سورية لليشمانية المدارية. مجلة التشخيص المخبري مج. 8، ع. 5 (كانون الثاني 2018).
https://search.emarefa.net/detail/BIM-835812
American Medical Association (AMA)
عرابي، أميرة ومحمد معروف والعموري، مصطفى. تقصي جين V-ATPase subunit C لدى سلالة سورية لليشمانية المدارية. مجلة التشخيص المخبري. 2018. مج. 8، ع. 5.
https://search.emarefa.net/detail/BIM-835812
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية
Record ID
BIM-835812