إعادة النظر بطريقة ARMS-PCR للتنميط الجيني ل STAT4 rs7574865 لدى مرضى التهاب مفاصل روماتويدي سوريين
Other Title(s)
Revisiting of ARMS-PCR method for STAT4 rs7574865 genotyping in Syrian rheumatoid arthritis patients
Joint Authors
Source
Issue
Vol. 8, Issue 5 (31 Jan. 2018)9 p.
Publisher
Syrian Clinical Laboratory Association
Publication Date
2018-01-31
Country of Publication
Syria
No. of Pages
9
Main Subjects
Abstract EN
Single Nucleotide Polymorphisms (SNPs) are becoming favored genetic markers.
A large variety of the techniques have been developed for screening this type of mutations.
But mostly, these techniques are expensive and require specialized equipments.
In our study, we worked on evaluating ARMS-PCR (Amplification Refractory Mutation System-Polymerase Chain Reaction) method.
The optimization process was done using genomic DNA samples from rheumatoid arthritis patients to detect the genotype STAT4 rs7574865 (G/T).
The study included 13 DNA samples, whose STAT4 rs7574865 genotypes were already known using DNA sequencing method.
The optimization was done using different primers concentrations and annealing temperatures.
The optimized ARMS-PCR reaction was applied to the samples, but two of them showed a different genotype from the genotype shown by sequencing reaction.
Our study has set the thermal conditions and primer concentrations for the ARMS-PCR reaction, for the genotyping STAT4 rs7574865 polymorphism, but it still needs some modifications to attain more specificity.
ARMS-PCR is an easy, applicable and not costly method.
American Psychological Association (APA)
طرقجي، إيمان ومنعم، فوزة. 2018. إعادة النظر بطريقة ARMS-PCR للتنميط الجيني ل STAT4 rs7574865 لدى مرضى التهاب مفاصل روماتويدي سوريين. مجلة التشخيص المخبري،مج. 8، ع. 5.
https://search.emarefa.net/detail/BIM-835823
Modern Language Association (MLA)
طرقجي، إيمان ومنعم، فوزة. إعادة النظر بطريقة ARMS-PCR للتنميط الجيني ل STAT4 rs7574865 لدى مرضى التهاب مفاصل روماتويدي سوريين. مجلة التشخيص المخبري مج. 8، ع. 5 (كانون الثاني 2018).
https://search.emarefa.net/detail/BIM-835823
American Medical Association (AMA)
طرقجي، إيمان ومنعم، فوزة. إعادة النظر بطريقة ARMS-PCR للتنميط الجيني ل STAT4 rs7574865 لدى مرضى التهاب مفاصل روماتويدي سوريين. مجلة التشخيص المخبري. 2018. مج. 8، ع. 5.
https://search.emarefa.net/detail/BIM-835823
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية
Record ID
BIM-835823