![](/images/graphics-bg.png)
A modified protocol for highly efficient EBV-mediated immortalization of human B lymphocytes from small volumes of peripheral blood serum
Author
Source
The Egyptian Journal of Medical Human Genetics
Issue
Vol. 19, Issue 3 (31 Jul. 2018), pp.221-223, 3 p.
Publisher
Egyptian Society of Human Genetics
Publication Date
2018-07-31
Country of Publication
Egypt
No. of Pages
3
Main Subjects
Abstract EN
Background : Many human molecular and genetic studies require the use of a renewable biological material.
Although primary fibroblast cell lines can be used for this purpose, there are disadvantages associated with human biopsies including the limited number of cell divisions.
Peripheral blood has the advantage of being easier to obtain but also the drawback that blood cells produce only short-term cultures.
Epstein-Barr virus is capable of transforming human B lymphocytes into indefinitely proliferating cells that can be maintained in tissue culture.
Here, we report a convenient method of B-lymphocyte immortalization using small volumes of freshly collected human blood serum saturated with nucleated blood cells.
Aim of the study: The aim of the present study is modification and improvement of the protocol for highly efficient immortalization of human B lymphocytes from small volume of blood samples.
Material and methods: Cell line B95-8 was used as Epstein-Barr virus source for viral stock preparation.
Immortalizing medium contains RPMI-1640, viral stock and additives.
No feeder layer was used.
Results: As result we present high efficient method for B lymphocytes immortalization with start blood volume less than 5 ml.
Conclusion: The method is applicable for immortalization of B lymphocytes from small blood samples and is essential for studies involving children or patients when large blood volume sampling is impossible.
American Psychological Association (APA)
Lemskaya, Natalya A.. 2018. A modified protocol for highly efficient EBV-mediated immortalization of human B lymphocytes from small volumes of peripheral blood serum. The Egyptian Journal of Medical Human Genetics،Vol. 19, no. 3, pp.221-223.
https://search.emarefa.net/detail/BIM-836376
Modern Language Association (MLA)
Lemskaya, Natalya A.. A modified protocol for highly efficient EBV-mediated immortalization of human B lymphocytes from small volumes of peripheral blood serum. The Egyptian Journal of Medical Human Genetics Vol. 19, no. 3 (Jul. 2018), pp.221-223.
https://search.emarefa.net/detail/BIM-836376
American Medical Association (AMA)
Lemskaya, Natalya A.. A modified protocol for highly efficient EBV-mediated immortalization of human B lymphocytes from small volumes of peripheral blood serum. The Egyptian Journal of Medical Human Genetics. 2018. Vol. 19, no. 3, pp.221-223.
https://search.emarefa.net/detail/BIM-836376
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 223
Record ID
BIM-836376