First detection of FOX-1 AmpC β-lactamase gene expression among Escherichia coli isolated from abattoir samples in Abakaliki, Nigeria
Joint Authors
Chika, Ejikeugwu
Charles, Esimone
Ifeanyichukwu, Iroha
Michael, Adikwu
Source
Issue
Vol. 33, Issue 3 (31 May. 2018), pp.134-140, 7 p.
Publisher
Publication Date
2018-05-31
Country of Publication
Oman
No. of Pages
7
Main Subjects
Abstract EN
Objectives: Gram-negative bacteria represent the most relevant reservoir of resistance to antibiotics in the environment.
The natural selection of resistant clones of bacteria in the environment by antimicrobial selective pressure is a relevant mechanism for spreading antibiotic resistance traits in both the community and hospital environment.
This is common in scenarios where antimicrobials are used irrationally, and even in the propagation of livestock, poultry birds, and for other veterinary purposes.
This study sought to detect the prevalence of FOX-1 AmpC β-lactamase genes from abattoir samples.
Methods: The isolation of Escherichia coli, antimicrobial susceptibility testing, and β-lactamase characterization was carried out using standard microbiology techniques.
The production of AmpC β-lactamase was phenotypically carried out using the cefoxitin-cloxacillin double-disk synergy test (CC-DD ST), and FOX-1 AmpC genes was detected in the E.
coli isolates using multiplex polymerase chain reaction.
Results: Forty-eight E.
coli isolates were recovered from the anal swabs of cows and 35 (72.9%) isolates were positive for the production of β-lactamase.
Notably, high percentages of resistance to cefoxitin (91.7%), ceftriaxone (83.3%), imipenem (85.4%), ceftazidime (87.5%), ofloxacin (81.3%), and gentamicin (85.4%) were found.
FOX-1 genes were detected in three (6.3%) of the 48 E.
coli isolates phenotypically screened for AmpC enzyme production.
Conclusions: Abattoirs could represent a major reservoir of resistance genes especially AmpC β-lactamase, and this could serve as a route for the dissemination of multidrug-resistant bacteria in the community.
Thus, the molecular identification of drug-resistant genes is vital for a reliable epidemiological investigation and the forestalling of the emergence and spread of these organisms through the food chain in this region
American Psychological Association (APA)
Chika, Ejikeugwu& Charles, Esimone& Ifeanyichukwu, Iroha& Michael, Adikwu. 2018. First detection of FOX-1 AmpC β-lactamase gene expression among Escherichia coli isolated from abattoir samples in Abakaliki, Nigeria. Oman Medical Journal،Vol. 33, no. 3, pp.134-140.
https://search.emarefa.net/detail/BIM-838235
Modern Language Association (MLA)
Chika, Ejikeugwu…[et al.]. First detection of FOX-1 AmpC β-lactamase gene expression among Escherichia coli isolated from abattoir samples in Abakaliki, Nigeria. Oman Medical Journal Vol. 33, no. 3 (May. 2018), pp.134-140.
https://search.emarefa.net/detail/BIM-838235
American Medical Association (AMA)
Chika, Ejikeugwu& Charles, Esimone& Ifeanyichukwu, Iroha& Michael, Adikwu. First detection of FOX-1 AmpC β-lactamase gene expression among Escherichia coli isolated from abattoir samples in Abakaliki, Nigeria. Oman Medical Journal. 2018. Vol. 33, no. 3, pp.134-140.
https://search.emarefa.net/detail/BIM-838235
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 139-140
Record ID
BIM-838235