Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA

Joint Authors

Zharikova, Natalia V.
Iasakov, Timur R.
Bumazhkin, Boris K.
Patutina, Ekaterina O.
Zhurenko, Evgeniia I.
Korobov, Vladislav V.
Sagitova, Alina I.
Kuznetsov, Boris B.
Markusheva, Tatiana V.

Source

Saudi Journal of Biological Sciences

Issue

Vol. 25, Issue 4 (31 May. 2018), pp.660-671, 12 p.

Publisher

Saudi Biological Society

Publication Date

2018-05-31

Country of Publication

Saudi Arabia

No. of Pages

12

Main Subjects

Biology

Topics

Abstract EN

A small plasmid designated pCS36-4CPA with a size of 5217 base pairs and G-C content of 50.74 % was isolated from Citrobacter sp.

36-4CPA.

The origin of replication (ori) of the plasmid was identified as a region of about 800 bp in length with an identity of 67.1 % to the ColE1 plasmid at the nucleotide level.

The replication region contained typical elements of ColE1-like plasmids: RNA I and RNA II with their corresponding _10 and _35 boxes, a single-strand initiation site (ssi), and a lagging-strand termination site (terH).

As seen in other ColE1-like plasmids, pCS36- 4CPA carried mobilisation machinery that include mobABCD genes but it did not possess the rom gene.

Analysis of the multimer resolution site (mrs) was performed and XerC and XerD binding sites were identified.

Also, the 70-nt transcript Rcd of pCS36-4CPA was predicted and similarity of the transcript’s secondary structure with those of the ColE1-family was shown.

The cargo module of pCS36-4CPA contained three open reading frames (ORFs).

Two of them (ORF5 and ORF6) showed no significant homology to any known gene sequences but contained putative THAP DNA-binding (DBD) and type II restriction endonuclease EcoO109I domains.

The seventh open reading frame (ORF7) encodes YhdJ-like DNA modification methylase.

The region highly homologous to pCS36-4CPA was found in the Salmonella phage SE2 genome.

American Psychological Association (APA)

Zharikova, Natalia V.& Iasakov, Timur R.& Bumazhkin, Boris K.& Patutina, Ekaterina O.& Zhurenko, Evgeniia I.& Korobov, Vladislav V.…[et al.]. 2018. Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA. Saudi Journal of Biological Sciences،Vol. 25, no. 4, pp.660-671.
https://search.emarefa.net/detail/BIM-838490

Modern Language Association (MLA)

Zharikova, Natalia V.…[et al.]. Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA. Saudi Journal of Biological Sciences Vol. 25, no. 4 (May. 2018), pp.660-671.
https://search.emarefa.net/detail/BIM-838490

American Medical Association (AMA)

Zharikova, Natalia V.& Iasakov, Timur R.& Bumazhkin, Boris K.& Patutina, Ekaterina O.& Zhurenko, Evgeniia I.& Korobov, Vladislav V.…[et al.]. Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 4, pp.660-671.
https://search.emarefa.net/detail/BIM-838490

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 669-671

Record ID

BIM-838490