Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA
Joint Authors
Zharikova, Natalia V.
Iasakov, Timur R.
Bumazhkin, Boris K.
Patutina, Ekaterina O.
Zhurenko, Evgeniia I.
Korobov, Vladislav V.
Sagitova, Alina I.
Kuznetsov, Boris B.
Markusheva, Tatiana V.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 4 (31 May. 2018), pp.660-671, 12 p.
Publisher
Publication Date
2018-05-31
Country of Publication
Saudi Arabia
No. of Pages
12
Main Subjects
Topics
Abstract EN
A small plasmid designated pCS36-4CPA with a size of 5217 base pairs and G-C content of 50.74 % was isolated from Citrobacter sp.
36-4CPA.
The origin of replication (ori) of the plasmid was identified as a region of about 800 bp in length with an identity of 67.1 % to the ColE1 plasmid at the nucleotide level.
The replication region contained typical elements of ColE1-like plasmids: RNA I and RNA II with their corresponding _10 and _35 boxes, a single-strand initiation site (ssi), and a lagging-strand termination site (terH).
As seen in other ColE1-like plasmids, pCS36- 4CPA carried mobilisation machinery that include mobABCD genes but it did not possess the rom gene.
Analysis of the multimer resolution site (mrs) was performed and XerC and XerD binding sites were identified.
Also, the 70-nt transcript Rcd of pCS36-4CPA was predicted and similarity of the transcript’s secondary structure with those of the ColE1-family was shown.
The cargo module of pCS36-4CPA contained three open reading frames (ORFs).
Two of them (ORF5 and ORF6) showed no significant homology to any known gene sequences but contained putative THAP DNA-binding (DBD) and type II restriction endonuclease EcoO109I domains.
The seventh open reading frame (ORF7) encodes YhdJ-like DNA modification methylase.
The region highly homologous to pCS36-4CPA was found in the Salmonella phage SE2 genome.
American Psychological Association (APA)
Zharikova, Natalia V.& Iasakov, Timur R.& Bumazhkin, Boris K.& Patutina, Ekaterina O.& Zhurenko, Evgeniia I.& Korobov, Vladislav V.…[et al.]. 2018. Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA. Saudi Journal of Biological Sciences،Vol. 25, no. 4, pp.660-671.
https://search.emarefa.net/detail/BIM-838490
Modern Language Association (MLA)
Zharikova, Natalia V.…[et al.]. Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA. Saudi Journal of Biological Sciences Vol. 25, no. 4 (May. 2018), pp.660-671.
https://search.emarefa.net/detail/BIM-838490
American Medical Association (AMA)
Zharikova, Natalia V.& Iasakov, Timur R.& Bumazhkin, Boris K.& Patutina, Ekaterina O.& Zhurenko, Evgeniia I.& Korobov, Vladislav V.…[et al.]. Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 4, pp.660-671.
https://search.emarefa.net/detail/BIM-838490
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 669-671
Record ID
BIM-838490