microRNA-1297 involves in the progression of oral squamous cell carcinoma through PTEN

Abstract EN

Background: Oral squamous cell carcinoma (OSCC) oncogenic mechanisms still remain elusive.

Herein, we proposed to understand the biological role of a newly discovered OSCC miRNA.

Methods: MiR-1297 related to OSCC was selected for this study.

TaqMan qRT- PCR assay was used to profile miRNA and gene expression in 16 tumors with matched adjacent non-tumor tissues.

CCK-8 assay and colony formation assay were used to demonstrate cell proliferation.

Protein level was determined by western blotting.

SPSS was used for statistical analyses.

Results: MiR-1297 is repressed and PTEN activated in OSCC.

Moreover, the miR-1297 overexpressing cell lines displayed a decrease in cell growth rate.

And, suppression of miR-1297 reversed the cell growth rate.

In addition, PTEN silencing display the similar pattern as miRNA-1297 overexpression to enhance OSCC cell growth.

Conclusions: MiRNA and gene expression changes are common event in OSCC.

Our results suggest miR- 1297 may drive tumor progression through PTEN.

And, miR-1297 could be a promising candidate for future investigation.