Upregulation of miR-582-5p regulates cell proliferation and apoptosis by targeting AKT3 in human endometrial carcinoma
Joint Authors
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 5 (31 Jul. 2018), pp.965-970, 6 p.
Publisher
Publication Date
2018-07-31
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Abstract EN
The human endometrial carcinoma is one of the most common female malignancies, and there is an urgent requirement to explore new therapeutic strategies.
There is accumulating evidence that microRNAs (miRNAs) can serve as potential diagnostic and prognostic biomarkers for various types of cancer, but the significance of miR-582-5p still remains largely unknown in the endometrial carcinoma.
The aims of this study were to understand and identify the influence of miR-582-5p on the proliferation and apoptosis of human endometrial carcinoma and its relevant mechanism.
First, quantitative real-time PCR (qRT-PCR) was used to detect miR-582-5p and AKT3 expression in human tissue samples and cells.
Then, CyQuant assay and 2D colony assay were employed to evaluate cell proliferation.
Western blotting was used to determine protein expression.
Subsequently, the luciferase reporter assay was used to identify the target of miR-582-5p.
Finally, Annexin V assay was used to detect cell apoptosis.
We found that miR-582-5p expression was significantly decreased in human endometrial carcinoma tissues, and miR-582-5p upregulation in human endometrial carcinoma cells inhibit cell proliferation and promote apoptosis.
Moreover, AKT3 was validated as a target of miR-582-5p and AKT3 expression was inversely correlated with miR-582-5p expression.
Besides, AKT3 upregulation efficiently abrogates the effect of miR-582-5p on the cells.
These results demonstrated that miR-582-5p regulates cell proliferation and apoptosis in human endometrial carcinoma via AKT3.
Thus, miR-582-5p represents a potential therapeutic target in human endometrial carcinoma meriting further investigation
American Psychological Association (APA)
Li, Lingling& Ma, Li. 2018. Upregulation of miR-582-5p regulates cell proliferation and apoptosis by targeting AKT3 in human endometrial carcinoma. Saudi Journal of Biological Sciences،Vol. 25, no. 5, pp.965-970.
https://search.emarefa.net/detail/BIM-838780
Modern Language Association (MLA)
Li, Lingling& Ma, Li. Upregulation of miR-582-5p regulates cell proliferation and apoptosis by targeting AKT3 in human endometrial carcinoma. Saudi Journal of Biological Sciences Vol. 25, no. 5 (Jul. 2018), pp.965-970.
https://search.emarefa.net/detail/BIM-838780
American Medical Association (AMA)
Li, Lingling& Ma, Li. Upregulation of miR-582-5p regulates cell proliferation and apoptosis by targeting AKT3 in human endometrial carcinoma. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 5, pp.965-970.
https://search.emarefa.net/detail/BIM-838780
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 969-970
Record ID
BIM-838780