Evaluation of antioxidants and reactive oxygen species (ROS)‎ levels after combination exposure to chromium (III)‎ and atrazine on liver in wister albion male rats

Abstract EN

Atrazine (ATZ)is remaining as one of the most widely broad spectrum pesticide used to control on annual grasses and broadleaf weeds.

While Chromium Cr (III) it has been used currently as food supplemented.

The current study was designed to determine impact of Cr at 7,30 and 300ppm on liver was in absence or in the present of (25 and 50mg/kg) of ATZ for 6 weeks in rats.

Results indicated that deficiency or supplementation with Cr did not cause measurable toxicity and has no obvious effect on oxidative stress or anti-oxidant enzymes and lipid metabolism.

Furthermore, the results showed that the mixture increased level of Aryl Hydrocarbon Receptor (AhR), Reactive Oxygen Species (ROS).

Moreover, the mixture had effects on antioxidants such as super oxide dismutase (SOD), Catalase (CAT), Reduced glutathione (GSH), activity where demonstrated more sever in liver tissue compared to Cr alone, especially as dose of ATZ increased.

Moreover, as dose of Cr in the mixture was increased, the ROS, AhR, CAT, GSH activity was increased compared to low Cr values.

However, SOD activity showed reduction in elevation of mixture concentrations.

The results of this study suggest that there is synergic impact of co-exposure to ATZ and Cr on the parameters used as markers for liver toxicity in the current study.

Further studies are warranted to investigate the interact of Cr and ATZ toxicity in more detail and to avoid having the Cr supplement where ATZ exposure is identified.