Effect of some vitamin D receptor gene variants on the frequency of sister chromatid exchange in Iraqi women with polycystic ovary syndrome

Abstract EN

Polycystic ovary syndrome (PCOS) is among the most common disorders of premenopausal women , cause infertility.

This study was conducted to investigate whether the vitamin D receptor (VDR) gene polymorphisms are associated with the enhancement of sister chromatid exchanges(SCE) .

This study was carried out in the Institute of Genetic Engineering and Biotechnology for Postgraduate Studies - University of Baghdad through the period from 1 November 2016 until the end of August 2017.

The PCOS samples were taken from the Kamal Al-Samarraee infertility treatment Hospital in Bag hdad.

The genomic instability were evaluated by measured the frequency of sister chromatid exchange in the presence of 5-bromodeoxy- uridine (BrdU) and using the Hoechst stain in 32 PCOS patients who have homozygous mutant result in genotyping assay , and from 34 PCOS patients who have heterozygous result assay.

as well as 14 apparently healthy women of 50 women as control group.

PCOS are classified depending on the results of the genotyping assay.

The result showed significant differences in the level of sister chromatid exchange in patients with PCOS for each of VDR gene polymorphisms compared with normal women.

This study concluded that the SCE as affected by polycystic ovary syndrome and the genotypes of VDR gene.