Sequence analysis of sub-genotype D hepatitis B surface antigens isolated from Jeddah, Saudi Arabia
Joint Authors
al-Haddad, Sahar
al-Akilli, Salihah
Rabah, Samar
Sabir, Jamal
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 4 (31 May. 2018), pp.838-847, 10 p.
Publisher
Publication Date
2018-05-31
Country of Publication
Saudi Arabia
No. of Pages
10
Main Subjects
Abstract EN
Little is known about the prevalence of HBV genotypes/sub-genotypes in Jeddah province, although the hepatitis B virus (HBV) was identified as the most predominant type of hepatitis in Saudi Arabia.
To characterize HBV genotypes/sub-genotypes, serum samples from 15 patients with chronic HBV were collected and subjected to HBsAg gene amplification and sequence analysis.
Phylogenetic analysis of the HBsAg gene sequences revealed that 11 (48 %) isolates belonged to HBV/D while 4 (18 %) were associated with HBV/C.
Notably, a HBV/D sub-genotype phylogenetic tree identified that eight current isolates (72 %) belonged to HBV / D1, whereas three isolates (28 %) appeared to be more closely related to HBV / D5, although they formed a novel cluster supported by a branch with 99 % bootstrap value.
Isolates belonging to D1 were grouped in one branch and seemed to be more closely related to various strains isolated from different countries.
For further determination of whether the three current isolates belonged to HBV/D5 or represented a novel sub-genotype, HBV / DA, whole HBV genome sequences would be required.
In the present study, we verified that HBV / D1 is the most prevalent HBV sub-genotype in Jeddah, and identified novel variant mutations suggesting that an additional sub-genotype designated HBV / DA should be proposed.
Overall, the results of the present HBsAg sequence analyses provide us with insights regarding the nucleotide differences between the present HBsAg / D isolates identified in the populace of Jeddah, Saudi Arabia and those previously isolated worldwide.
Additional studies with large numbers of subjects in other areas might lead to the discovery of the specific HBV strain genotypes or even additional new sub-genotypes that are circulating in Saudi Arabia.
American Psychological Association (APA)
al-Haddad, Sahar& al-Akilli, Salihah& Rabah, Samar& Sabir, Jamal. 2018. Sequence analysis of sub-genotype D hepatitis B surface antigens isolated from Jeddah, Saudi Arabia. Saudi Journal of Biological Sciences،Vol. 25, no. 4, pp.838-847.
https://search.emarefa.net/detail/BIM-838864
Modern Language Association (MLA)
al-Haddad, Sahar…[et al.]. Sequence analysis of sub-genotype D hepatitis B surface antigens isolated from Jeddah, Saudi Arabia. Saudi Journal of Biological Sciences Vol. 25, no. 4 (May. 2018), pp.838-847.
https://search.emarefa.net/detail/BIM-838864
American Medical Association (AMA)
al-Haddad, Sahar& al-Akilli, Salihah& Rabah, Samar& Sabir, Jamal. Sequence analysis of sub-genotype D hepatitis B surface antigens isolated from Jeddah, Saudi Arabia. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 4, pp.838-847.
https://search.emarefa.net/detail/BIM-838864
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 846-847
Record ID
BIM-838864