Extraction and characterization of listeria monocytogenes exopolysaccharide

Other Title(s)

استخلاص و توصيف متعدد السكريات الخارجي لجراثيم Listeria monocytogenes

Joint Authors

al-Abbasi, Raghad Riyad Shafiq
al-Sanjary, Sahirah Idris Hamid

Source

Rafidain Journal of Science

Issue

Vol. 27, Issue 2 (30 Apr. 2018), pp.23-31, 9 p.

Publisher

University of Mosul College of Science

Publication Date

2018-04-30

Country of Publication

Iraq

No. of Pages

9

Main Subjects

Agriculture

Abstract EN

This study was aimed to extract exopolysaccharide (EPS) produced by Listeria monocytogenes and investigate the maximum production by using different culture media and various factors.

The EPS was obtained from the culture supernatant by cold ethanol precipitation and yield maximum production (0.368mg /ml ) in basal salt solution (BSS) as dry weight.

The total carbohydrate content was determined by phenol sulfuric acid method.

The functional groups were detected by Fourier transform infrared (FTIR) spectroscopy at frequency range of 400- 4000cm-1.

Different alcohols were checked for their efficiency on precipitating EPS, those other than ethanol, methanol and isopropanol were able to sediment the EPS, while isoamylalcohol show no effect on precipitation.The results also indicated that the medium volume play an important role in EPS production.

American Psychological Association (APA)

al-Abbasi, Raghad Riyad Shafiq& al-Sanjary, Sahirah Idris Hamid. 2018. Extraction and characterization of listeria monocytogenes exopolysaccharide. Rafidain Journal of Science،Vol. 27, no. 2, pp.23-31.
https://search.emarefa.net/detail/BIM-838935

Modern Language Association (MLA)

al-Abbasi, Raghad Riyad Shafiq& al-Sanjary, Sahirah Idris Hamid. Extraction and characterization of listeria monocytogenes exopolysaccharide. Rafidain Journal of Science Vol. 27, no. 2 (2018), pp.23-31.
https://search.emarefa.net/detail/BIM-838935

American Medical Association (AMA)

al-Abbasi, Raghad Riyad Shafiq& al-Sanjary, Sahirah Idris Hamid. Extraction and characterization of listeria monocytogenes exopolysaccharide. Rafidain Journal of Science. 2018. Vol. 27, no. 2, pp.23-31.
https://search.emarefa.net/detail/BIM-838935

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 30-31

Record ID

BIM-838935