Extraction and characterization of listeria monocytogenes exopolysaccharide
Other Title(s)
استخلاص و توصيف متعدد السكريات الخارجي لجراثيم Listeria monocytogenes
Joint Authors
al-Abbasi, Raghad Riyad Shafiq
al-Sanjary, Sahirah Idris Hamid
Source
Issue
Vol. 27, Issue 2 (30 Apr. 2018), pp.23-31, 9 p.
Publisher
University of Mosul College of Science
Publication Date
2018-04-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
This study was aimed to extract exopolysaccharide (EPS) produced by Listeria monocytogenes and investigate the maximum production by using different culture media and various factors.
The EPS was obtained from the culture supernatant by cold ethanol precipitation and yield maximum production (0.368mg /ml ) in basal salt solution (BSS) as dry weight.
The total carbohydrate content was determined by phenol sulfuric acid method.
The functional groups were detected by Fourier transform infrared (FTIR) spectroscopy at frequency range of 400- 4000cm-1.
Different alcohols were checked for their efficiency on precipitating EPS, those other than ethanol, methanol and isopropanol were able to sediment the EPS, while isoamylalcohol show no effect on precipitation.The results also indicated that the medium volume play an important role in EPS production.
American Psychological Association (APA)
al-Abbasi, Raghad Riyad Shafiq& al-Sanjary, Sahirah Idris Hamid. 2018. Extraction and characterization of listeria monocytogenes exopolysaccharide. Rafidain Journal of Science،Vol. 27, no. 2, pp.23-31.
https://search.emarefa.net/detail/BIM-838935
Modern Language Association (MLA)
al-Abbasi, Raghad Riyad Shafiq& al-Sanjary, Sahirah Idris Hamid. Extraction and characterization of listeria monocytogenes exopolysaccharide. Rafidain Journal of Science Vol. 27, no. 2 (2018), pp.23-31.
https://search.emarefa.net/detail/BIM-838935
American Medical Association (AMA)
al-Abbasi, Raghad Riyad Shafiq& al-Sanjary, Sahirah Idris Hamid. Extraction and characterization of listeria monocytogenes exopolysaccharide. Rafidain Journal of Science. 2018. Vol. 27, no. 2, pp.23-31.
https://search.emarefa.net/detail/BIM-838935
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 30-31
Record ID
BIM-838935