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Heat shock stimulate division of hypocotyle suspension culture of dianthus caryophyllus L.
Author
Source
al-Tarbiyah wa-al-Ilm : Majallat ilmiyah lil-Buhuth al-Ilmiyah al-Asasiyah
Issue
Vol. 27, Issue 2 (30 Jun. 2018), pp.39-47, 9 p.
Publisher
University of Mosul College of Education for Pure Science
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
The study succeeded in establishing a fine cell suspension derived from callus of carnation, Dianthus caryophyllus L.
Hypocotyle explants in liquid MS medium supplemented with 0.5 mgL-1 2,4-D and 0.1 mgL-1 BA.
The cells begin its first division within 24 h.
These cell were continued their subsequent divisions to produce 142x103 cell/ml culture after five days of initiation.
Results indicated that exposure of cell suspension to 20, 25, 30, 35, 40, 45, and 50oC short-term 5, 10 min.
and long-term 15-20 min.
stimulate the division of cells suspension in all treatments.
Heat shock sustained the division of cell cultured in multiple drop array (MDA) technique and increase the formation of cellular colonies which produced a numerous of calli primordia.
The total number of colonies was 959 developed when cells exposed to with the short-term 35oC/5 min heat shock.
The produced calli was green in colour and friable in texture.
American Psychological Association (APA)
Zaydan, Sahlah Muhammad. 2018. Heat shock stimulate division of hypocotyle suspension culture of dianthus caryophyllus L.. al-Tarbiyah wa-al-Ilm : Majallat ilmiyah lil-Buhuth al-Ilmiyah al-Asasiyah،Vol. 27, no. 2, pp.39-47.
https://search.emarefa.net/detail/BIM-838942
Modern Language Association (MLA)
Zaydan, Sahlah Muhammad. Heat shock stimulate division of hypocotyle suspension culture of dianthus caryophyllus L.. al-Tarbiyah wa-al-Ilm : Majallat ilmiyah lil-Buhuth al-Ilmiyah al-Asasiyah Vol. 27, no. 2 (2018), pp.39-47.
https://search.emarefa.net/detail/BIM-838942
American Medical Association (AMA)
Zaydan, Sahlah Muhammad. Heat shock stimulate division of hypocotyle suspension culture of dianthus caryophyllus L.. al-Tarbiyah wa-al-Ilm : Majallat ilmiyah lil-Buhuth al-Ilmiyah al-Asasiyah. 2018. Vol. 27, no. 2, pp.39-47.
https://search.emarefa.net/detail/BIM-838942
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 46-47
Record ID
BIM-838942