In vitro cysteine reactivates organophosphate insecticide dichlorvos-inhibited human cholinesterases
Other Title(s)
الإضافة للسيستين يعيد تنشيط أنزيم كولينستراز البشري التي يثبطها مبيد الحشرات العضوية الفوسفاتية الديكلوروفوس
Joint Authors
Muhammadi, Hamid Rida
Jaliliyan, Jafar
Karimi, Muhammad Yahya
Shitab Bu Shihri, Sayyid V.
Source
Sultan Qaboos University Medical Journal
Issue
Vol. 17, Issue 3 (31 Aug. 2017), pp.293-300, 8 p.
Publisher
Sultan Qaboos University College of Medicine and Health Sciences
Publication Date
2017-08-31
Country of Publication
Oman
No. of Pages
8
Main Subjects
Abstract EN
Objectives : Organophosphate (OP) pesticides inhibit both red blood cell (RBC) and plasma cholinesterases (ChEs).
Oximes, especially pralidoxime (2-PAM), are widely used as antidotes to treat OP poisoning.
In addition, N-acetylcysteine (NAC) is sometimes used as an adjuvant antidote.
The current study aimed to assess the feasibility of using NAC as a single therapeutic agent for OP poisoning in comparison to in vitro 2-PAM.
Methods: This study was carried out at the Razi Drug Research Center of Iran University of Medical Sciences, Tehran, Iran, between April and September 2014.
A total of 22 healthy human subjects were recruited and 8 mL citrated blood samples were drawn from each subject.
Dichlorvos-inhibited blood samples were separately exposed to low and high doses (final concentrations of 300 and 600 μmol.L-1, respectively) of 2-PAM, NAC and cysteine.
Plasma and RBCs were then separated by centrifugation and their ChE activity was measured using spectrophotometry.
Results : Although cysteine—and not NAC—increased the ChE activity of both plasma and RBCs over those of dichlorvos, it did not increase them over those of a high dose of 2-PAM.
Conclusion: These results suggest that the direct reactions of 2-PAM and cysteine with dichlorvos and the reactivation of phosphorylated ChEs occurr via an associative stepwise addition-elimination process.
High therapeutic blood concentrations of cysteine are needed for the elevation of ChE activity in plasma and RBCs; however, both this agent and NAC may still be effective in the reactivation of plasma and RBC ChEs.
American Psychological Association (APA)
Muhammadi, Hamid Rida& Jaliliyan, Jafar& Karimi, Muhammad Yahya& Shitab Bu Shihri, Sayyid V.. 2017. In vitro cysteine reactivates organophosphate insecticide dichlorvos-inhibited human cholinesterases. Sultan Qaboos University Medical Journal،Vol. 17, no. 3, pp.293-300.
https://search.emarefa.net/detail/BIM-839746
Modern Language Association (MLA)
Muhammadi, Hamid Rida…[et al.]. In vitro cysteine reactivates organophosphate insecticide dichlorvos-inhibited human cholinesterases. Sultan Qaboos University Medical Journal Vol. 17, no. 3 (Aug. 2017), pp.293-300.
https://search.emarefa.net/detail/BIM-839746
American Medical Association (AMA)
Muhammadi, Hamid Rida& Jaliliyan, Jafar& Karimi, Muhammad Yahya& Shitab Bu Shihri, Sayyid V.. In vitro cysteine reactivates organophosphate insecticide dichlorvos-inhibited human cholinesterases. Sultan Qaboos University Medical Journal. 2017. Vol. 17, no. 3, pp.293-300.
https://search.emarefa.net/detail/BIM-839746
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 299-300
Record ID
BIM-839746