Morphological changes in a human retinal pigment epithelial cell line following infection by Toxoplasma gondii
Other Title(s)
دراسة التغيرات الشكلية للخلايا الطلائية لمبطنة لحاجز للعين عند الإصابة بطفيلي التوكسوبلازما
Author
al-Sandaqshi, Ala Tariq Abd al-Wahid
Source
Basrah Journal of Veterinary Research
Issue
Vol. 16, Issue 2 (31 Dec. 2017), pp.36-44, 9 p.
Publisher
University of Basrah College of Veterinary Medicine
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
Retinitis is the most important clinical consequences of Toxoplasmosis but the mechanisms used by T.
gondii tachyzoites to invade the retina are not yet understood.
In the current study, cellular pathogenicity of Toxoplasmosis in a human retinal pigment epithelial cell line (ARPE-19) was investigated.
Following in vitro infection of ARPE-19 with T.
gondii tachyzoites, cell viability associated with infection was identified by Methyl thiazolyl tetrazolium (MTT) assay.
Intracellular development of T.
gondii tachyzoites within ARPE-19 was characterized by acridine orange (AO) staining.
Morphological changes associated with infection was assessed by electron microscopy (EM).
The results obtained showed that ARPE-19 permit the invasion, growth and development of T.
gondii tachyzoites and that infection can cause a metabolic burden on the host cells and multiple morphological changes in the relocation of the host cell organelles (mitochondria, endoplasmic reticulum, and Golgi apparatus) around the parasitphorous vacuole.
American Psychological Association (APA)
al-Sandaqshi, Ala Tariq Abd al-Wahid. 2017. Morphological changes in a human retinal pigment epithelial cell line following infection by Toxoplasma gondii. Basrah Journal of Veterinary Research،Vol. 16, no. 2, pp.36-44.
https://search.emarefa.net/detail/BIM-840098
Modern Language Association (MLA)
al-Sandaqshi, Ala Tariq Abd al-Wahid. Morphological changes in a human retinal pigment epithelial cell line following infection by Toxoplasma gondii. Basrah Journal of Veterinary Research Vol. 16, no. 2 (2017), pp.36-44.
https://search.emarefa.net/detail/BIM-840098
American Medical Association (AMA)
al-Sandaqshi, Ala Tariq Abd al-Wahid. Morphological changes in a human retinal pigment epithelial cell line following infection by Toxoplasma gondii. Basrah Journal of Veterinary Research. 2017. Vol. 16, no. 2, pp.36-44.
https://search.emarefa.net/detail/BIM-840098
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 43-44
Record ID
BIM-840098