Allelic variations of dehydrin genes in the second mutant generation M2 of some durum wheat genotypes

Abstract EN

This study was conducted at the Biotechnology Laboratory, Department of Agronomy, Faculty of Agriculture, Damascus University (Syria) and the laboratories.

of National Commission for Biotechnology in the year 2012.

The aim of the studywas to detect the variations of Dehydrin in different genotypes of durum wheat in the second mutant generation (M2).

Results of dehydrin gene (responsible for drought tolerance) variation study showed a clear difference among the studied treatments.

Variation in the molecular weight between loci per gene was very high in some cases, while it had a high degree of symmetry in other cases, and was easily distinguished on 4% agarose gel.

The PCR results for the Dehydrin genes Dhn1, Dhn3, Dhn6 and Dhn10 showed a monomorphic pattern in most of the studied treatments, while for the Dhn12 only one pattern was found in one treatment (medium concentration of DES in Bouhouth9).

Dhn2 showed seven patterns, while the genes Dhn4, Dhn8 and Dhn11 showed five patterns, and the Dhn7 showed fourpatterns and finally Dhn9 showed three patterns.

The Dhn11 was superior in the number of polymorphic patterns, as the number of total patterns was 46 patterns in all treatments, but on the other hand the Dhn12 showed the lowest number of patterns with only one pattern.

The Dhn5 didn’t show any patterns in all cases, the treatment (v 25 kr) in Bouhouth9 showed the largest number of patterns in the Dhn genes with 18 patterns, and the treatment (DES 0.05%) in Douma1 showed the lowest number with only 10patterns.