Effect of Ursolic acid, hyperinsulinemia and vitamin B complex on some biochemical parameters and skeletal muscle histology of Alloxan induced diabetic rabbits

Abstract EN

This study aimed to evaluate the effect of ursolic acid, hyperinsulinemia and vitamin B complex in skeletal muscle, which represented by Gastrocnemius muscle after sciatic nerve crush in diabetes mellitus male rabbits, determine their effects on some biochemical parameters which include insulin, total serum protein (TSP) and skeletal muscle protein percentage and detect their effects on histological structures of the skeletal muscle represented by Gastrocnemius muscle.

The results of our study showed that there is a significant increase of insulin in diabetic rabbits and this increase is continued in all treatment groups with a higher level range of (3.56-8.4 μu/ ml); the lowest significant increase is with vitamin B complex treated group.

Our study illustrated that Induction of diabetes has no significant effect on skeletal muscle protein percentage but has a significant effect on total serum protein, while all treatment groups caused a significant increase in skeletal muscle protein percentage and total serum protein.

Histological sections of the Gastrocnemius muscle represent some changes happened due to sciatic nerve crush injury, such as some distraction in skeletal muscle fibers, splitting and atrophy of others as well as there is an increase in thickness of fibrous tissue separating them.

There is a good regeneration of muscle fibers with little splitting phenomena; also there is a clear formation of muscle cell nuclei, which indicate an improvement of muscle after different treatment.