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Enhanced differentiation of wharton’s jelly-derived mesenchymal stem cells in insulin-producing cells by the extract of nigella sativa seeds
Joint Authors
Abediankenari, Said
Amiri, Marzei Mumin inezhad
Ranjbaran, Husayn
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 20, Issue 3 (31 Mar. 2018), pp.1-6, 6 p.
Publisher
Publication Date
2018-03-31
Country of Publication
United Arab Emirates
No. of Pages
6
Main Subjects
Abstract EN
Background: With regards to the high potential of medicinal plants in the production of biopharmaceuticals, one can rely on the promising prospect of insulin production via plant resources.
Objectives: This study was conducted with the aim of using plant extract for insulin-producing cells.
Methods: This was a quasi-experimental study using critical case sampling.
Six samples were gathered from the umbilical cord (Wharton’s jelly) in a governmental university affiliated hospital, Sari, Iran in 2017 after successful isolation of mesenchymal stem cells.
Initially, Nigella sativa seeds extraction was performed to prepare the extract for cellular differentiation.
Next, dithizone (DTZ) staining was used to evaluate insulin production, and insulin level was examined by the enzyme-linked immunosorbent assay (ELISA).
Data were analyzed was analyzed with the SPSS version 16 software using independent sample t-test.
Results: Themeanof theamountof insulin secretionwas92.335.13 ng/ml for the interventionand0.330.15 ng/ml for the control group.
The results showed that there was a significant difference in the average insulin in the culture obtained from Nigella sativa seeds between control and intervention groups (P = 0.001).
In addition, via the ELISA kit and specific dithizone staining, insulinproducing cells were proven.
Conclusions: In this regard, it could be concluded that the extract of Nigella sativa seeds was capable of including differentiation of mesenchymal stem cells derived from Wharton’s jelly to Insulin-Producing Cells.
American Psychological Association (APA)
Ranjbaran, Husayn& Abediankenari, Said& Amiri, Marzei Mumin inezhad. 2018. Enhanced differentiation of wharton’s jelly-derived mesenchymal stem cells in insulin-producing cells by the extract of nigella sativa seeds. Iranian Red Crescent Medical Journal،Vol. 20, no. 3, pp.1-6.
https://search.emarefa.net/detail/BIM-840297
Modern Language Association (MLA)
Ranjbaran, Husayn…[et al.]. Enhanced differentiation of wharton’s jelly-derived mesenchymal stem cells in insulin-producing cells by the extract of nigella sativa seeds. Iranian Red Crescent Medical Journal Vol. 20, no. 3 (Mar. 2018), pp.1-6.
https://search.emarefa.net/detail/BIM-840297
American Medical Association (AMA)
Ranjbaran, Husayn& Abediankenari, Said& Amiri, Marzei Mumin inezhad. Enhanced differentiation of wharton’s jelly-derived mesenchymal stem cells in insulin-producing cells by the extract of nigella sativa seeds. Iranian Red Crescent Medical Journal. 2018. Vol. 20, no. 3, pp.1-6.
https://search.emarefa.net/detail/BIM-840297
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6
Record ID
BIM-840297