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Modified dot elisa for diagnosis of zoonotic toxoplasmosis in man and sheep with clinical suggestive manifestations
Joint Authors
al-Bahi, Muhammad M.
Mahrus, Liliyan N.
Abd al-Rahman, Ghadah H.
Junaydi, Mursi Ratib
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 47, Issue 3 (31 Dec. 2017), pp.679-688, 10 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2017-12-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Fractionation of Toxoplasma gondii RH strain tachyzoites (TTAg) using SDS-PAGE and identification of its specific bands versus control sera using EITB revealed five group of specific fractions corresponding to molecular weight (MW) level of 32-34 kda, 46kda, 53 kda, 78 kda and 100 kda.
Fractions of 32-34 kda were the most sensitive and specific one able to capturing very low level of anti-Toxoplasma Ab in the sera and did not cross react with anti-parasites antibodies (Ab) as hydatedosis, S.
mansoni, HCV, F.
gigantica and Cysticercus ovies as well as control noninfected sera.
Thick transverse gel strip containing the fraction of 32-34kda were cut out, its contents were eluted and concentrated, Dotting of 2μl from this fraction on 4mm diameter nitrocellulose (NC) discs for development a modified dot-ELISA technique were evaluated in diagnosis of toxoplasmosis in group of suspected women and sheep.
This technique succeeded in diagnosis of the specific anti-toxoplasma Ab in sera of seven suspected sheep, all of them aborted after this.
Investigation of some haematological and serum chemical parameters demonstrated significant (p≤0.05) deviations in the values of WBCs, AST, Urea and Cholesterol, which considered to be important in supporting early diagnoses of infection in suspected cases.
Adaptation of modified Dot-ELISA technique described in this work perform an easily field applied mass screening technique depends on using a minute amount of serum without special equipment.
Dot-ELISA by this way gathering the benefits of accuracy of EITB and overcome the cross reaction phenomenon characteristic to ELISA.
This improved capability of early diagnosis and control strategy of toxoplasmosis in man and animals
American Psychological Association (APA)
al-Bahi, Muhammad M.& Junaydi, Mursi Ratib& Mahrus, Liliyan N.& Abd al-Rahman, Ghadah H.. 2017. Modified dot elisa for diagnosis of zoonotic toxoplasmosis in man and sheep with clinical suggestive manifestations. Journal of the Egyptian Society of Parasitology،Vol. 47, no. 3, pp.679-688.
https://search.emarefa.net/detail/BIM-840919
Modern Language Association (MLA)
al-Bahi, Muhammad M.…[et al.]. Modified dot elisa for diagnosis of zoonotic toxoplasmosis in man and sheep with clinical suggestive manifestations. Journal of the Egyptian Society of Parasitology Vol. 47, no. 3 (Dec. 2017), pp.679-688.
https://search.emarefa.net/detail/BIM-840919
American Medical Association (AMA)
al-Bahi, Muhammad M.& Junaydi, Mursi Ratib& Mahrus, Liliyan N.& Abd al-Rahman, Ghadah H.. Modified dot elisa for diagnosis of zoonotic toxoplasmosis in man and sheep with clinical suggestive manifestations. Journal of the Egyptian Society of Parasitology. 2017. Vol. 47, no. 3, pp.679-688.
https://search.emarefa.net/detail/BIM-840919
Data Type
Journal Articles
Language
English
Notes
Includes appendix : p. 688
Record ID
BIM-840919