Prevalence, risk factors and comparative diagnostic study between immunofluorescence assay and ordinary staining techniques in detection of blastocystis hominis in fecal samples
Joint Authors
Abd al-Al, Najla Fathi
Farghali, Adil
Hamzah, Raniya Said
Mutawalli, Samirah
Faraj, Shayma Muhammad
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 47, Issue 3 (31 Dec. 2017), pp.701-708, 8 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2017-12-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Blastocytis hominis (B.
hominis) is a prevalent protozoon parasite.
Difficulties in diagnostic approach to detect this parasite are still present.
Moreover, the data on its prevalence are missing in many locations and inhabitants.
This study compared between the sensitivity of immunofluorescence assay (IFA) and ordinary staining methods; Iodine, Safranine Methylene Blue (SMB), Modified Ziehl Neelsen (MZN) and Trichrome stains to identify B.
hominis infection in fecal samples and its prevalence in Zagazig City.
Full history taking and clinical examination were done for 201 cases of both sexes aged (2-50) years.
Stool samples were examined using Iodine, SMB, MZN, Trichrome and IFA stains.
B.
hominis was detected in 85 cases (42.3%), of which 52 cases were B.
hominis alone and 32 cases were asymptomatic (61.5%).
The highest prevalence (49.4%) was detected among (2-15years) age group mostly males.
IFA stain was taken as the gold standard.
The sensitivity of trichrome, MZN, iodine, SMB stains were 91.7%, 72.9%, 60%, 55.3% respectively, and specificity was 100% for all stains
American Psychological Association (APA)
Farghali, Adil& Hamzah, Raniya Said& Abd al-Al, Najla Fathi& Mutawalli, Samirah& Faraj, Shayma Muhammad. 2017. Prevalence, risk factors and comparative diagnostic study between immunofluorescence assay and ordinary staining techniques in detection of blastocystis hominis in fecal samples. Journal of the Egyptian Society of Parasitology،Vol. 47, no. 3, pp.701-708.
https://search.emarefa.net/detail/BIM-840934
Modern Language Association (MLA)
Farghali, Adil…[et al.]. Prevalence, risk factors and comparative diagnostic study between immunofluorescence assay and ordinary staining techniques in detection of blastocystis hominis in fecal samples. Journal of the Egyptian Society of Parasitology Vol. 47, no. 3 (Dec. 2017), pp.701-708.
https://search.emarefa.net/detail/BIM-840934
American Medical Association (AMA)
Farghali, Adil& Hamzah, Raniya Said& Abd al-Al, Najla Fathi& Mutawalli, Samirah& Faraj, Shayma Muhammad. Prevalence, risk factors and comparative diagnostic study between immunofluorescence assay and ordinary staining techniques in detection of blastocystis hominis in fecal samples. Journal of the Egyptian Society of Parasitology. 2017. Vol. 47, no. 3, pp.701-708.
https://search.emarefa.net/detail/BIM-840934
Data Type
Journal Articles
Language
English
Notes
Includes appendix : p. 708
Record ID
BIM-840934