وجود فيروسات البطاطس البطاطا في مناطق الزراعات الرئيسة في السعودية
Other Title(s)
Occurrence of potato viruses in the major potato growing areas in Saudi Arabia
Source
Issue
Vol. 36, Issue 2 (31 Aug. 2018), pp.114-122, 9 p.
Publisher
Arab Society for Plant Protection
Publication Date
2018-08-31
Country of Publication
Lebanon
No. of Pages
9
Main Subjects
Abstract EN
Potato (Solanum tuberosum L.) is the fourth largest staple food crop worldwide after wheat, maize, and rice.
In Saudi Arabia, potato is cultivated during the spring and fall growing seasons.
Viruses are the most devastating pathogens on potato, and the most widespread viruses on potatoes around the world are Potato leaf roll virus (PLRV, genus Polerovirus, family Luteoviridae), Potato virus Y (PVY, genus Potyvirus, family Potyviridae), Potato virus A (PVA, genus Potyvirus, family Potyviridae), Potato virus M (PVM, genus Carlavirus, family Flexiviridae), Potato virus S (PVS, genus Carlavirus, family Flexiviridae) and Potato virus X (PVX, genus Potexvirus, family Flexiviridae).
Field inspection of potato fields was carried out in 6 regions, Hail, Hard, Jouf, Qaseem, Riyadh and Tabuk.
A total of 1524 samples that showed symptoms of virus infection such as rolling, mosaic, stunting, yellowing, leaf curl, and vein necrosis were collected from all regions.
All samples were initially tested using ELISA and selected samples were tested by RT- PCR.
Results obtained indicated that PVY was the most detected virus followed by AMV and PLRV.
The RT-PCR test was more accurate and sensitive than ELISA.
All primer pairs for RT-PCR were used under similar conditions, and the annealing temperature of 58°C gave the best amplification products for all primers used in this study.
RT-PCR conditions described in this study are considered useful, sufficient, applicable, and effective and allow simultaneous detection of the four potato viruses in one-step RT-PCR
American Psychological Association (APA)
الهديب، خالد بن عبد الله. 2018. وجود فيروسات البطاطس البطاطا في مناطق الزراعات الرئيسة في السعودية. مجلة وقاية النبات العربية،مج. 36، ع. 2، ص ص. 114-122.
https://search.emarefa.net/detail/BIM-841403
Modern Language Association (MLA)
الهديب، خالد بن عبد الله. وجود فيروسات البطاطس البطاطا في مناطق الزراعات الرئيسة في السعودية. مجلة وقاية النبات العربية مج. 36، ع. 2 (2018)، ص ص. 114-122.
https://search.emarefa.net/detail/BIM-841403
American Medical Association (AMA)
الهديب، خالد بن عبد الله. وجود فيروسات البطاطس البطاطا في مناطق الزراعات الرئيسة في السعودية. مجلة وقاية النبات العربية. 2018. مج. 36، ع. 2، ص ص. 114-122.
https://search.emarefa.net/detail/BIM-841403
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 121-122
Record ID
BIM-841403