وجود فيروسات البطاطس البطاطا في مناطق الزراعات الرئيسة في السعودية

Abstract EN

Potato (Solanum tuberosum L.) is the fourth largest staple food crop worldwide after wheat, maize, and rice.

In Saudi Arabia, potato is cultivated during the spring and fall growing seasons.

Viruses are the most devastating pathogens on potato, and the most widespread viruses on potatoes around the world are Potato leaf roll virus (PLRV, genus Polerovirus, family Luteoviridae), Potato virus Y (PVY, genus Potyvirus, family Potyviridae), Potato virus A (PVA, genus Potyvirus, family Potyviridae), Potato virus M (PVM, genus Carlavirus, family Flexiviridae), Potato virus S (PVS, genus Carlavirus, family Flexiviridae) and Potato virus X (PVX, genus Potexvirus, family Flexiviridae).

Field inspection of potato fields was carried out in 6 regions, Hail, Hard, Jouf, Qaseem, Riyadh and Tabuk.

A total of 1524 samples that showed symptoms of virus infection such as rolling, mosaic, stunting, yellowing, leaf curl, and vein necrosis were collected from all regions.

All samples were initially tested using ELISA and selected samples were tested by RT- PCR.

Results obtained indicated that PVY was the most detected virus followed by AMV and PLRV.

The RT-PCR test was more accurate and sensitive than ELISA.

All primer pairs for RT-PCR were used under similar conditions, and the annealing temperature of 58°C gave the best amplification products for all primers used in this study.

RT-PCR conditions described in this study are considered useful, sufficient, applicable, and effective and allow simultaneous detection of the four potato viruses in one-step RT-PCR