Antitumor activity of L-asparaginase produced from E. coli.

Other Title(s)

الفعالية المضادة للأورام لإنزيم ل-أسباراجينيز المنتج من بكتيريا القولون

Author

al-Rawi, Khalid Faruq

Source

Iraqi Journal of Cancer and Medical Genetics

Issue

Vol. 10, Issue 1 (30 Jun. 2017), pp.78-87, 10 p.

Publisher

al-Mustansyriah University Iraqi Centre for Cancer and Medical Genetics Research

Publication Date

2017-06-30

Country of Publication

Iraq

No. of Pages

10

Main Subjects

Medicine

Abstract EN

.Extra and intra-cellular L-asparaginase were produced from E.coli, purified by ammonium sulphate precipitation, ion exchange chromatography and gel filtration chromatography and tested for their anti-tumor activities against four human tumor cell lines include MCF-7 , PC3 , A549 and Hep-G2 using MTT and (HCS) array scan.

The MTT assay showed that the enzymes exhibited antiproliferative activity in different cell lines growth, PC3 cell line were more sensitive to extra and intracellular L-asparaginase as it gave a lower IC50 value (27.41±2.95 and 70.12±2.53) for extra and intracellular respectively, extracellular L-asparaginase treatment increases nuclear condensation, plasma membrane permeability, MMP attenuation and increased cytochrome C in the cystol compared to control.

We suggest the extra-cellular Lasparaginase of E.coli was a therapeutic agent against human PC3 cell line.

American Psychological Association (APA)

al-Rawi, Khalid Faruq. 2017. Antitumor activity of L-asparaginase produced from E. coli.. Iraqi Journal of Cancer and Medical Genetics،Vol. 10, no. 1, pp.78-87.
https://search.emarefa.net/detail/BIM-844637

Modern Language Association (MLA)

al-Rawi, Khalid Faruq. Antitumor activity of L-asparaginase produced from E. coli.. Iraqi Journal of Cancer and Medical Genetics Vol. 10, no. 1 (2017), pp.78-87.
https://search.emarefa.net/detail/BIM-844637

American Medical Association (AMA)

al-Rawi, Khalid Faruq. Antitumor activity of L-asparaginase produced from E. coli.. Iraqi Journal of Cancer and Medical Genetics. 2017. Vol. 10, no. 1, pp.78-87.
https://search.emarefa.net/detail/BIM-844637

Data Type

Journal Articles

Language

English

Notes

Record ID

BIM-844637