Molecular detection of invA gene for Salmonella spp. isolates from poultry in Babil province-Iraq

Abstract EN

A total of 150 poultry samples (age from 1 to 49 day) were collected from different locations at Babylon province ) Al-hashimiya , Al-madhatiya and Al-Qasim ) from November 2015 to April 2016.

These samples were collected from different part of the body (Liver tissue, Yolk sac content, and cecal swab).

Salmonella spp.

was isolated and identified using bacterial culturing on selective media, in addition to, biochemical and Mini API 20E and serotyping by monovalent antisera.

Polymerase chain reaction (PCR) was used to detect invA of Salmonella spp.

The results revealed that the rate of Salmonella isolates from poultry specimens were (11) 7.3% using cultural and biochemical tests, the results of serotyping revealed these isolates belong to Salmonella spp.

The PCR technique was used to detect invA gene, these Salmonella isolates appeared to contain this gene since DNA amplification showed one distinct band (size 389 bp) when electrophoresed on agarose gel.

The results of this study revealed that the PCR technique had a high specific in detection of Salmonella spp.

When compared with other conventional detection methods