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Molecular detection and phylogenetic analysis of Coxiella burnetii in goats milk
Author
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 16, Issue 1 (30 Jun. 2017), pp.75-78, 4 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
4
Main Subjects
Abstract EN
Over the last years, the number Coxiella burnetii infections increased throughout the wourld.
A molecular detection and phylogenetic analysis were performed in Al-Diwaniayh city in which the first phylogenetic documentation of C.burnetii reported in this area with accession numbers KY576802, KY576803, KY576804 and KY576805.
Molecular detection and phylogenetic analysis of C.burnetii targeting the transposase gene partial sequence was done on the milk samples from 50 apperently healthy goats.
DNA sequence was amplified in 6 out of 50 (12%) milk samples collected from different regions of Al Diwaniya city.
Four of these amplicons were submitted to sequences analysis and gave 99% nucleotides sequence similarity.
Also the amplicon sequences of local strain were compared with global C.burnetti sequences in NCBI which revealed close related to NCBI-Blast C.burnetii transposase gene of Indian (AB84899.1) and Brazilian (JF970261.1) strains, whereas there was genetic variation to NCBI-Blast C.burnetii transposase gene for USA (DQ379976.1), Portugal (EU009657.1) and Taiwan (EU000273.1).
This work confirm goats infection and shedding the C.
burnetii in Al-Diwaniayh, Iraq and determined the phylogenetic tree of local strain of this bacterium.
American Psychological Association (APA)
Ayiz, Haydar Naji. 2017. Molecular detection and phylogenetic analysis of Coxiella burnetii in goats milk. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 16, no. 1, pp.75-78.
https://search.emarefa.net/detail/BIM-844832
Modern Language Association (MLA)
Ayiz, Haydar Naji. Molecular detection and phylogenetic analysis of Coxiella burnetii in goats milk. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 16, no. 1 (2017), pp.75-78.
https://search.emarefa.net/detail/BIM-844832
American Medical Association (AMA)
Ayiz, Haydar Naji. Molecular detection and phylogenetic analysis of Coxiella burnetii in goats milk. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2017. Vol. 16, no. 1, pp.75-78.
https://search.emarefa.net/detail/BIM-844832
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 78
Record ID
BIM-844832