Improving conditions for gliotoxin production by local isolates of Aspergillus fumigatus
Other Title(s)
تحسين الظروف من عزلات محلية من الفطر Aspergillus fumigatus لإنتاج مستويات عالية من الكلايوتوكسين
Joint Authors
al-Azami, Abd al-Karim Jasim Hashim
Dhib, Batul Umran
Husayn, Anam Fuad
Sadiq, Sarah Haytham
Sulayman, Ghassan Muhammad
Source
Journal of Biotechnology Research Center
Issue
Vol. 11, Issue 2 (31 Dec. 2017), pp.14-24, 11 p.
Publisher
al-Nahrain University Biotechnology Research Center
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Abstract EN
Thirty two isolates of Aspergillus fumigatus were obtained from a total of 44 samples of sputum, nose swab and tracheal aspirate from suspected patient with aspergillosis were also collected from February 2014 to June 2014.
A morphological examination of A.
fumigatus was first made with naked eye and at low magnification power of microscope after that detailed examination was done by measuring the dimensions of the microscopic structures, photographing the microscopic structures and using relevant literature.
Results appeared conical-shaped terminal vesicles, uniseriate row of phialides on the upper two thirds of the vesicle, conidiophore stipes were short, phialides arrange uniseriate upper vesicle conidia and parallel to axis of conidiophore, produced in chains of spore basipetally from phialides, the chains of spore were borne directly in the absence of metulae and represented by septet and branching hyphae.
The ability of A.
fumigatus for GT production was investigated using thin layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) techniques and results showed that GT was produced by 81.25% of A.
fumigatus isolates.
Optimum conditions for GT production by A.
fumigatus 16 (AF-16) isolate were determined by submerged fermentation using Yeast extract sucrose medium.
Results indicated that AF-16 isolate was the highest GT producer on Yeast Exract Sucrose medium with inoculum size 2×107 conidium and incubation at 32ºC for 15 days and the concentration of gliotoxin was (4511µg mL-1).
American Psychological Association (APA)
Husayn, Anam Fuad& Sulayman, Ghassan Muhammad& Dhib, Batul Umran& al-Azami, Abd al-Karim Jasim Hashim& Sadiq, Sarah Haytham. 2017. Improving conditions for gliotoxin production by local isolates of Aspergillus fumigatus. Journal of Biotechnology Research Center،Vol. 11, no. 2, pp.14-24.
https://search.emarefa.net/detail/BIM-846999
Modern Language Association (MLA)
Sulayman, Ghassan Muhammad…[et al.]. Improving conditions for gliotoxin production by local isolates of Aspergillus fumigatus. Journal of Biotechnology Research Center Vol. 11, no. 2 (Dec. 2017), pp.14-24.
https://search.emarefa.net/detail/BIM-846999
American Medical Association (AMA)
Husayn, Anam Fuad& Sulayman, Ghassan Muhammad& Dhib, Batul Umran& al-Azami, Abd al-Karim Jasim Hashim& Sadiq, Sarah Haytham. Improving conditions for gliotoxin production by local isolates of Aspergillus fumigatus. Journal of Biotechnology Research Center. 2017. Vol. 11, no. 2, pp.14-24.
https://search.emarefa.net/detail/BIM-846999
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-846999