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Molecular detection of invA, ssaP in salmonella typhimurium isolated from chicken in al-Qadisiyah province
Joint Authors
Ismail, Jamilah Radi
Nimah, Azhar Abd al-Sadah
al-Ubaydi, Janan Nazim Sadiq
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 16, Issue 2 (31 Dec. 2017), pp.8-13, 6 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
Salmonella is considered the most important cause of foodborne diseases.
Identification of Salmonella typhimurium evaluated using bacteriological assay followed by PCR technique.
In this study, 40 intestinal content specimens of poultry were collected randomly from different farms of Al-Qadisiyah province.
Outs of 40 samples obtained, 14 isolates (35 %) were detected as Salmonella typhimurium according to conventional bacteriological characteristics, the Vitek 2 system for identification and molecular assays.
Two sets of primers were designed for detecting invA and ssaP genes.
These genes potent the virulence of Salmonella typhimurium.
The primers were made in this study by using NCBIGenBank and design online.
The primers were made by (Bioneer) company, Korea.
Molecular assay of the isolates gives away specific PCR products of 677bp for the invA gene and 314bp for ssaP gene.
The invA genes were amplified in 11 (78.5%) out of 14 isolates of Salmonella typhimurium, while ssaP genes were amplified in 10 (71.4%) out of 14 isolates of Salmonella typhimurium.
The result of the study confirms the ability of these specific primers for detection of salmonella typhimurium in samples of chicken as well as the rapidly and sensitivity of the PCR method as a good tool for bacteriological identification.
American Psychological Association (APA)
al-Ubaydi, Janan Nazim Sadiq& Ismail, Jamilah Radi& Nimah, Azhar Abd al-Sadah. 2017. Molecular detection of invA, ssaP in salmonella typhimurium isolated from chicken in al-Qadisiyah province. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 16, no. 2, pp.8-13.
https://search.emarefa.net/detail/BIM-847101
Modern Language Association (MLA)
al-Ubaydi, Janan Nazim Sadiq…[et al.]. Molecular detection of invA, ssaP in salmonella typhimurium isolated from chicken in al-Qadisiyah province. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 16, no. 2 (2017), pp.8-13.
https://search.emarefa.net/detail/BIM-847101
American Medical Association (AMA)
al-Ubaydi, Janan Nazim Sadiq& Ismail, Jamilah Radi& Nimah, Azhar Abd al-Sadah. Molecular detection of invA, ssaP in salmonella typhimurium isolated from chicken in al-Qadisiyah province. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2017. Vol. 16, no. 2, pp.8-13.
https://search.emarefa.net/detail/BIM-847101
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 12-13
Record ID
BIM-847101