Some techniques for sex election in Wistar rats

Abstract EN

The present study carried out on (36) mature female Wister rats about (65-70) days old divided into (6) groups included (6) female which not given any treatment as control group, other (30) female treated with progesterone (20 mg/kg, s.c) daily for 7 days , after 24hrs.

injected with PMSG (150 IU/kg, i.p) and after 48hrs.

injected LH (75 IU/kg, i.p) and divided for (5) groups within 14-15hrs.

equally, (T1) put with mature male rat (1:2) and isolated after 48hrs.

for natural mating, (T2) given alkaline solution in vagina for two days during first and second day from natural mating with mature male rat and removed after 48hrs., (T3) treated with acidic solution in vagina for two days during first and second day from natural mating with mature male rat and separated after 48hrs., (T4) inseminated artificially with upper layer of solution prepared from tail of epididymis and put with castrated male rat for 48hrs.

after injected with oxytocin (10 m IU/g, i.p), (T5)inseminated artificially with a lower layer of a solution prepared from a tail of epididymis and put with a castrated male for 48hrs.

after injection of oxytocin (10 m IU/g, i.p).

The results revealedT4& T2 recorded high ratio of newly born males (71.429%&63.415%), while (C, T1, T3& T5) were (28.571, 30.952, 23.810 and 25%) respectively.

While the high ratio of newly born females rate recorded in (T3&T5) were (76.190% and75%), and in (C, T1, T2,&T4) recorded (71.429, 69.048, 36.585 and 28.571%) respectively.

The pregnancy rate was 100% in all hormonally treated groups compared with the control group and groups inseminated artificially which recorded 50%.

Addition to that, the results showed highly significant variance (P≤0.01) in newly born numbers with a mathematic decrease in body weights of hormonally treated groups.