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Estimation of heat shock protein 70 (Hsp 70) gene expression in nile tilapia (Oreochromis niloticus) using quantitative realtime PCR
Other Title(s)
تقدير تعبير الجين المسئول عن بروتين الصدمة الحرارية 70 كيلودالتون في أسماك البلطي النيلي باستخدام ال PCR الكمي
Joint Authors
Yusuf, Muhammad Abu Bakr Hasan
Ahmad, Mahasin Sultan Sayyid
al-Sabah, Ahmad Husayni Abduh Muhammad
Shawqi, Ahmad Shawqi Hasan
Source
Zagazig Journal for Agricultural Researches
Issue
Vol. 44, Issue 3 (30 Jun. 2017), pp.1003-1015, 13 p.
Publisher
Zagazig University Faculty of Agriculture
Publication Date
2017-06-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Heat shock protein 70 (Hsp70) is one of family of proteins expressed in response to a wide range of biotic and abiotic stress conditions.
Quantitative real-time PCR (qRT-PCR) has been used to study the expression profiles of heat shock protein (Hsp70) gene in Nile tilapia, Oreochromis niloticus.
Young fish (30 g) were exposed to different degrees of heat (10, 15, 35, 39) and control (28°C) for 2 and 4 hr., followed by qRT-PCR of Hsp70 mRNA, using Beta actin (ß-Actin) as a reference gene and flourogenic dyes.
Expression of Hsp70 gene was different among fish tissues (muscles, gills and liver).
In all treatments the expression level detected in male were more than female fish.
The highest expression organs after 2 hr., was muscles in both fish sex but after 4 hr., liver recorded the highest expression value at cold shock where muscles recorded the highest expression at heat shock treatments in both fish sex.
American Psychological Association (APA)
al-Sabah, Ahmad Husayni Abduh Muhammad& Shawqi, Ahmad Shawqi Hasan& Ahmad, Mahasin Sultan Sayyid& Yusuf, Muhammad Abu Bakr Hasan. 2017. Estimation of heat shock protein 70 (Hsp 70) gene expression in nile tilapia (Oreochromis niloticus) using quantitative realtime PCR. Zagazig Journal for Agricultural Researches،Vol. 44, no. 3, pp.1003-1015.
https://search.emarefa.net/detail/BIM-848431
Modern Language Association (MLA)
al-Sabah, Ahmad Husayni Abduh Muhammad…[et al.]. Estimation of heat shock protein 70 (Hsp 70) gene expression in nile tilapia (Oreochromis niloticus) using quantitative realtime PCR. Zagazig Journal for Agricultural Researches Vol. 44, no. 3 (2017), pp.1003-1015.
https://search.emarefa.net/detail/BIM-848431
American Medical Association (AMA)
al-Sabah, Ahmad Husayni Abduh Muhammad& Shawqi, Ahmad Shawqi Hasan& Ahmad, Mahasin Sultan Sayyid& Yusuf, Muhammad Abu Bakr Hasan. Estimation of heat shock protein 70 (Hsp 70) gene expression in nile tilapia (Oreochromis niloticus) using quantitative realtime PCR. Zagazig Journal for Agricultural Researches. 2017. Vol. 44, no. 3, pp.1003-1015.
https://search.emarefa.net/detail/BIM-848431
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-848431