Study on virulence factors of Escherichia coli isolated from calves suffer from diarrhea
Other Title(s)
دراسة عوامل الضراوة لأشيرشيا كولاي المعزولة من العجول التي تعاني من الإسهال
Joint Authors
Muhammad, Ghadah Mahir Abd al-Hakim
Sultan, Siraj al-Din
Said, Ahmad Mustafa
Abd al-Azim, Muhammad Wail
Source
Assiut Veterinary Medical Journal
Issue
Vol. 63, Issue 154 (31 Jul. 2017), pp.113-121, 9 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2017-07-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Diarrhea is an important problem in young calves, especially in suckling beef calves.
The mostcommon cause of diarrhea in calves is E.coli.
The present study was undertaken to screen for presence of virulence factors associated with colibacillosis isolated from one hundred and twenty five fecal samples of calves suffer from diarrhea (<3monthes).
All samples were submitted for bacteriological examination, serotyping and virulence tests (Congo red test, serum resistant test, sereny test and haemolytic activity).
Finally molecular Identification by using Polymerase chain reaction (PCR) to detect shiga toxin (stx1, stx2) genes.
E.coli isolates were fifty eight isolates (46.4%).
The fecal samples were obtained a Twelve different serotype of E.coli (O26:H11, O91:H21, O103:H2, O111:H2, O15:H21, O146:H21, O8,O113:H4, O128:H2, O124, O121:H7 and O55:H7).
It was recorded forty two isolates (72.4%) were congo red positive, forty two isolates (72.4%) were serum resistant, eleven isolates (18.96%) made keratoconjunctivitis of Guinea pig and fourteen isolates (24.13%) had hemolytic activity.
PCR was performed to detect stx1 and stx2 genes on different serotypes of E.coli isolates.
The serotypes which positive to stx1 gene only were three (O8,O113:H4,O128:H2) (30%), two serotypes were positive to stx2 only (O15:H21,O146:H21) (20%), four serotypes were positive to both stx1 and stx2 (O26:H11, O91:H21, O103:H2,O111:H2) (40%) and O124 was negative for both stx1 and stx2(10%).
This result of E.coli strains isolated from diarrheic calves implies that these animals are an important reservoir of Shiga toxin Escherichia coli (STEC) strains that are potentially pathogenic toward farm animals.
American Psychological Association (APA)
Muhammad, Ghadah Mahir Abd al-Hakim& Abd al-Azim, Muhammad Wail& Sultan, Siraj al-Din& Said, Ahmad Mustafa. 2017. Study on virulence factors of Escherichia coli isolated from calves suffer from diarrhea. Assiut Veterinary Medical Journal،Vol. 63, no. 154, pp.113-121.
https://search.emarefa.net/detail/BIM-848742
Modern Language Association (MLA)
Muhammad, Ghadah Mahir Abd al-Hakim…[et al.]. Study on virulence factors of Escherichia coli isolated from calves suffer from diarrhea. Assiut Veterinary Medical Journal Vol. 63, no. 154 (Jul. 2017), pp.113-121.
https://search.emarefa.net/detail/BIM-848742
American Medical Association (AMA)
Muhammad, Ghadah Mahir Abd al-Hakim& Abd al-Azim, Muhammad Wail& Sultan, Siraj al-Din& Said, Ahmad Mustafa. Study on virulence factors of Escherichia coli isolated from calves suffer from diarrhea. Assiut Veterinary Medical Journal. 2017. Vol. 63, no. 154, pp.113-121.
https://search.emarefa.net/detail/BIM-848742
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-848742