Prevalence of some antimicrobial resistance genes in multidrug resistant salmonella isolated from broiler chickens

Abstract EN

Salmonellosis is one of the major bacterial problems in the poultry industry in Egypt and worldwide.

Resistance to antimicrobial agents within nontyphoidal Salmonellae is a serious problem.

The present study aimed to analyze some β-lactamase resistance genes in Salmonella isolates from broiler chicken.

Five hundred samples were collected from diseased broiler chickens of different ages (3-6 weeks) from different farms in Assiut Governorate during the period from January 2015 to December 2015.

Bacteriological examination showed that 26 salmonella isolates were recovered with a prevalence rate of 5.2% Serotyping of Salmonella isolates showed that S.

Enteritidis S.

Typhimurium, and S.

Kentuky were identified at rates of 50%, 30.8% and 19.2%, respectively.

Results of antibiogram showed that 18 salmonella isolates (92.3%) were multidrug resistant.

All isolates were screened for the presence of 2 β-lactamase resistance genes (blaCTX and blaCMY) using multiplex PCR.

The overall prevalence was 14/26 (53.9%) for blaCTX and 9/26 (34.6%) for blaCMY.